查询词典 clones

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

The result showed 41 clones distribute in 26 OTUs, which exist in Acidimicrobidae and 7 suborders of Actinobacteridae respectively, especially the dominant community of Yutian pit was streptosporangineae instead of streptomycete, which accounting for 42.3% of sequenced clones.

结果表明， 41个克隆序列属于26个OTUs，分别分布于放线菌门放线菌亚纲的7个亚目和酸微菌亚纲，其中链孢囊菌亚目(Streptosporangi- neae)中放线菌组成丰富，占到了全部挑选克隆的42.3%，是于田盐池放线菌群落中的优势菌，而链霉菌不是高盐环境放线菌的优势菌群。

The results showed that the 16S rDNA copies of Bacteroides spp. decreased in rotavirus-infected children by 2 orders of magnitudes. 665 clones in Group H and 284 clones in Group R were sequenced and 34 OTUs were obtained according to 98% sequence homology. Partial least squares discriminant analysis of the clone library profiling data revealed significant structural shifts of gut microbiota.

利用偏最小二乘判别法(Partial least squares discriminant analysis，PLS-DA)对这34个OTU进行分析，结果表明轮状病毒感染导致肠道内拟杆菌组成结构发生了变化，通过拟杆菌的组成可以将健康个体与轮状病毒感染个体分开，而对于区分两组个体起关键作用的OTU分别与Bacteroides vulgatus ，Bacteroides stercoris和Bacteroides fragilis有很高的同源性。

Two of the six clones had the same DNA sequence,so there are five different antigenic epitopes in the six clones;the Blast analysis showed that there were no sequence homology between the five epitopes and the known antigen of paragonimus.

上述6个克隆有2个克隆的DNA序列完全相同，即6个克隆包含了5种不同的抗原表位；Blast分析表明5个表位与已知的肺吸虫抗原表位无DNA序列的同源性。

The individual growth,morphology and population biomass of Pennisetum centrasiaticum clones at two different habitats of farmland-sand dune ecotone on Keerqin sandy land of Inner Mongolia were compared in this study.P.centrasiaticum clones had significant morphological plasticity and different biomass distribution pattern at different habitats.

对生长在不同沙地生境下的白草无性系进行了个体生长与形态以及种群生物量等指标的比较研究。结果表明，生长在不同生境下的白草无性系表现出显著的形态可塑性和不同的生物量分配模式。

Under light, middle and heavy degradation, with the increase of degradation, the vegetative ramet capacity of the clones increased, while their reproductive ramet capacity decreased. A typical landscape of "black soil beach" appeared in grassland with extreme degradation; the growth of the clones was in a lowest level.

在轻度、中度和重度退化程度下，随着退化程度的增加，高山嵩草无性系营养分株能力加强，生殖分株能力减弱；极度退化的草地已呈现典型的&黑土滩&景观，高山嵩草的克隆生长维持在最低水平。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。