查询词典 clones

Had reached 20% of Macintosh unit sales, while the value of the Mac market had fallen 11%.15 Convinced that clones were cannibalizing Apple's sales, Jobs refused to license the latest Mac OS.

已达到20 ％的麦金塔单位销售，而价值的Mac市场已经下降11 ％， 0.15深信克隆拆苹果的销售，乔布斯拒绝许可的最新的Mac OS 。

Such notable professionals as John Rivers hybridized various tree fruits, and introduced superior clones from France and elsewhere.

著名的专业工作者如John Rivers对各种果树进行了杂交，并从法国和其他地方引进优良的无性系。

For Star Wars: The Clone Wars, Bradley finds himself performing an altogether new concept of "range"-- voicing all of the clones and imbuing them with unique identities and slight variations.

在《星球大战：克隆人战争》中，布莱德利发现自己对表演的&戏路&有了一个全新的概念——给所有的克隆人配音，并且给他们注入独特的个性以及少许的变化。

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此，为了获得基因重组NT-3，研究其生物效应，本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体，并利用脂质体介导，转染进CHO细胞；经G418筛选，建立了中国仓鼠卵巢细胞系，稳定表达重组大鼠NT-3。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

Cut-and-paste tools include all lassos, marquees, clones, drags , and other repositioning tools.

剪切和粘贴工具包括所有的套索，如选取框、克隆、拖动及其他重定位工具。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

对其中一个克隆M79的DNA序列和蛋白质序列分析表明，M79基因编码多肽含有一个典型的MADS box和K-box，与植物中的许多MADS box基因产物有很高的同源性。

At the same time,some ovule-specific cDNA clones in Orchid and Petunia flowers had been isolated and among them MADS Box genes may be responsible for the ovule differentiation and formation.

同时，已分别从蝶兰和矮牵牛的胚珠中分离出一批胚珠发育特异的基因，其中有关MADS Box 基因在胚珠形成和发育中的作用研究得比较清楚，基因转化工作证实胚珠的分化和形成受一类新的MADS Box基因控制。

In Attack of the Clones, Bail Organa is played by Jimmy Smits.

在《克隆人的进攻》中，贝尔·奥加纳由吉米·斯米茨扮演。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。