查询词典 clones

As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome-based physical map of the Oryza sativa japonica Nipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O.

国际水稻基因组测序计划(International Rice Genome Sequencing Project)采用以物理图为基础的随机测序战略，作为该计划的一部分，中国科学院国家基因中心承担了水稻第四号染色体精确序列的测定任务。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

The clones generated by Thrawn were based on approximately 20 templates.

索龙的克隆人的生产是基于大约20个模板的。

One of the 17 positive clones we have obtained encoded the glutamine-rich carboxyl terminus of TIAR, and this interaction between tTG and TIAR, which was finely regulated by Ca 2+, was proved in vitro by GS...

序列测定显示其中 1个克隆所含cDNA序列编码TIA 1相关蛋白(TIA 1 relatedprotein ，TIAR )C端 12 9个氨基酸残基序列，GST下拉实验也证实tTG与TIAR能相互作用，而且这种相互作用需要Ca2 +参与作用。这些结果提示tTG可能通过其Ca2 +依赖的转谷氨酰胺活性对TIAR进行修饰从而影响TIAR的功能，可能在细胞凋亡中发挥着一定的作用。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

In this paper, 12 years old Chinese Fir clones were studied on the genetic variation of growth properties, basic density and tracheid morphological characteristics. The main results were as follows.

本文综合研究了12年生杉木无性系试验林的生长性状、基本密度和管胞形态的遗传变异规律。

The young -mature forest correlation in wood density,tracheid length, tracheid width is not notable in clones and its offspring of clonal seed orchards.

管胞长度与管胞宽度相关在家系间和无性系间都达到了显著水平。

A strain NK13 was screened for a certain extent asymmetric hydrolyze the rac-ketoprofen Chloroethyl ester and identified as Bacillus megaterium. For the preparation of gene libraries, a positive clones was obtained from the tributyrin flat. The sequence of this esterase gene had been analysised, and contained the whole ORF of an esterase gene with the length of 933bp.

以本实验室筛选出的一株具有不对称拆分消旋酮基布洛芬氯乙酯的菌株NK13为材料，经初步鉴定为巨大芽孢杆菌，通过构建其基因文库，从中筛选得到一阳性克隆重组子pUC-NK。

Flow cytometry analysis was used to evaluate cells surface antigen expression and cells binding Ulex europaeus agglutinin-1 of the early and late clones in group B was detected by direct fluorescence staining.

结果：早期克隆数目的获得在2组间差异无统计学意义，但序列黏附法获得晚期克隆数目增加（P〈0.05），且B组的早期克隆细胞表达CD3减少（P〈0.05）。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐