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Patients with clinical virologic breakthrough during NA therapy and 4 negative control patients without virologic breakthrough were selected for the study of quasispecies dynamics. All the 70 serum samples from these 10 patients were analyzed with the method of'PCR-cloning-sequencing'. Totally over 2,100 clones were sequenced. The target sequences obtained were aligned, compared and analyzed to study the mechanism of NA drug resistance and to understand the clinical course of NA therapy from a viral populational genetic point of view.

我们进一步采用国际认可的&金标准&&PCR-克隆-测序-序列分析&的方法，对6例发生临床病毒学突破的患者以及4例未发生临床病毒学突破的患者的全程血清样本进行了准种检测，共测序2100余个，以系统深入地对LAM和ETV治疗过程中HBV种群的演变及其临床意义进行探讨。

The description in growth characters and genetic variation of 7 new clones of Populus Deltoides is given in this paper.

本文对美洲黑杨7个新无性系在水培条件下的生长特性和遗传变异规律详细地进行了描述。

In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene, designated as Cpcor413pm1, was obtained by sequencing the randomly selected clones, on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis.

为研究蜡梅冬季开花过程的抗寒分子机理，在构建蜡梅花cDNA文库及EST分析的基础上，通过随机克隆测序，克隆了1个蜡梅COR413蛋白的cDNA基因，命名为Cpcor413pm1。

In peripheral blood obtained up to 77 months before a CLL diagnosis, prediagnostic B-cell clones were present in 44 of 45 patients with CLL.

45例白血病患者中，44例在CLL诊断前77个月内获得的外周血中发现了诊断前B细胞克隆。

RESULTS: On the basis of either flow-cytometric or molecular analysis, 44 of 45 patients with CLL (98%; 95% confidence interval , 88 to 100) had a prediagnostic B-cell clone; in 41 patients (91%; 95% CI, 79 to 98), the presence of the B-cell clone was confirmed by both methods. The presence of immunoglobulin heavy-chain variable genes was determined in 35 of 45 prediagnostic clones (78%).

结果：在流式细胞或分子分析的基础上， 45名白血病患者有44例（98 ％； 95 ％可信区间，88～100 ）具有诊断前B细胞克隆；在41例（ 91％； 95 ％可信区间， 79～98 ），B细胞克隆的存在得到了这两种方法的证实。45个诊断前克隆中有35个(78%)具有免疫球蛋白重链可变基因。

Using six-color flow cytometry (with antibodies CD45, CD19, CD5, CD10, kappa, and lambda) and immunoglobulin heavy-chain gene rearrangement by reverse-transcriptase-polymerase-chain-reaction assay, we determined the association between MBL and subsequent CLL and characterized the immunoglobulin gene repertoire of the prediagnostic B-cell clones.

使用六色流式细胞术（使用CD45、CD19、CD5、CD10抗体、Ig κ和Igλ）和逆转录聚合酶链反应试验的免疫球蛋白重链基因重排，我们确定了MBL和随后的CLL之间的关系，明确了诊断前B细胞克隆的免疫球蛋白基因库。

This paper reports the restriction allele frequencies, polymorphism information contents and heterozygote frequencies of the restriction sites of D4S10 locus, pK082/Hind Ⅲ(2),pK083/EcoRI(1) and pK081/EcoRI(2), detected by three sub-clones of the G8 probe and tries to do presymptomatic diagnosis of Huntington 's disease for phenotypically normal family members in two HD families.

报道53例样本D4S10基因座由G8探针3个亚探针检出的限制酶切位点pK082／HindⅢ（2）、pK083／EcoRⅠ（1）和pK081／EcoRⅠ（2）的等位片段频率、多态性信息含量和杂合子频率，并试对2个Huntington舞蹈病家系成员作症状前诊断。

Curiously, the look is not one of style as much as degree. There is no army of winsomely tousled RPattz clones. Instead, there are Afros, mohawks, dreadlocks and pompadours. There are "Idol"-ized punky pincushions, Allman-esque hippie cascades and Bowie-style, parti-colored shags. And these are just the styles that have names.

奇怪的是，这种头型几乎算不上一种严格意义上的风格，主要包括非洲式，莫霍克式，拉斯塔法里式和后梳式，并没有大量对RPattz蓬松式的模仿，这里有&偶像&式的朋克针垫，奥曼式嬉皮士瀑布头以及包威式和色彩斑斓的烟丝式，这些还是有名字的。

Totally 78 clones which were up-regulated and putatively involved in the defense response of G. barbadense were identified and sequenced. Sequence similarity searches were performed with the Blastn and Blastx.

对78个在海岛棉的抗病反应中呈上升表达的克隆进行了测序并对测序结果去载体后在NCBI上利用 Blastn和Blastx进行序列相似性分析。

Taking root suckers of Hippophae rhamnoides artificial population as the research object, based on the field investigation of produced number of clones, space distribution and growth condition, basic characteristics of ramet and relationship of environmental factors and root suckers were studied systematically in different habitats in Zhifanggou hilly-gully regions.

以沙棘人工种群根蘖萌苗为研究对象，通过对丘陵沟壑区纸坊沟流域不同生境沙棘人工种群根蘖萌苗，产生的无性系个数、空间分布和生长状况的调查，系统研究不同生境条件下沙棘人工种群的根系产生幼苗的基本特征及其与环境因素的关系。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。