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Results showed that microbial diversity in indole acclimated community was higher. Clones (73%) in quinoline acclimated community were Thauera related OTUs from the Rhodocyclaceae family. But OTUs from the families of Comamonadaceae, Alcaligenaceae and Rhodocyclaceae were dominant OTUs in indole acclimated community. The most dominant OTU from Comamonadaceae was 28.7％ in clone library.

两个群落的优势OTU也不同，喹啉驯化群落中Thauera及其它Rhodocyclaceae科的微生物占整个群落的73%，而吲哚驯化群落中优势OTU为Comamonadaceae科、Alcaligenaceae科和Rhodocyclaceae科等类型的微生物，其中Comamonadaceae科的一个OTU占整个群落的28.7%。

Among 90 positive clones in the 16S rDNA library of microorganisms, 23 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, delta subclasses of the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria and Planctomycetes.

采用限制性内切酶HaeIII和RsaI对克隆文库中的90个克隆子进行了酶切分型，根据ARDRA酶切图谱的不同，可将其分为23个OTUs.16S rDNA序列分析结果表明，该克隆文库中主要包括变形菌门的alpha、beta、detla亚类、厚壁菌门、放线菌门、拟杆菌门、酸杆菌门及浮霉菌门等8类细菌及未培养细菌。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

The recombinant pIND-ndr2vector and pIND vector were tranfected into GLC-82cells respectively,and the cell clones were screened with G418and zeocin.

连有ndr2基因的载体（pIND-ndr2）用脂质体方法转染到培养的肺腺癌细胞GLC-82中。

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

Recent efforts in California with clones of this variety are very promising, producing medium-bodied reds with rich cherry or plumlike flavors and aromas.

最近的努力， 1857年无性系品种，这是非常有前途的，生产中型健全红人丰富樱桃或plumlike风味和口感。

Pombe. An active recombination of pREP3X-HA-mid2 was identified from 41 single clones of relevant transformed S.

在41个酵母转化单菌落中，筛选到一个有表达活性的pREP3X-HA-mid2重组子。

An active recombination of pREP3X-HA-spm1 was identified from 35 single clones of relevant transformed S. pombe.

在35个酵母转化单菌落中，筛选到一个有表达活性的pREP3X-HA-spm1重组子。

Popular clones in California include: 777, 667, 115, 114, Pommard (Davis clone 5), Dijon, and Martini.

热门无性加州包括：777、667、115、114、pommard(戴维斯克隆5)、第戎、而努。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！