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Direction as theory of modren foresty and biotechnology, Based on the Breeding of superior clones, culture medium prescription of tissue culture and photoautotrophic micropropagation for the main species of industrial raw material forest and flower of Fujian were selected, and the sivilcuture model for the main species of industrial raw material forest were comfirmed, and the assistant Forest Management System for the main species of industrial raw material forest and flower of Fujian were established.

以现代林业理论及生物技术为理论指导，在优良无性系选育的基础上，筛选出相思树种等我省工业原料林主要树种及花卉优良品种组织培养、光自养植物微繁技术的培养基配方及轻质专用容器苗基质；确定了环境友好型的工业原料林主要树种栽培模式，建立了相思树种等我省工业原料林辅助经营管理系统。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Second, a lot of artificial propagation experiments were performed between different gynogenetic clones to analyze whether or not genetic recombination occurs by utilizing the identified RAPD and SCAR markers. Direct molecular genetic evidence for gonochoristic reproduction was revealed in the gynogenetic silver crucian carp, and a previous hypothesis that the silver crucian carp might reproduce both gynogenetically and gonochoristically was conformed.

二是利用银鲫的克隆多样性和其种群中存在的雄性个体，设计大量的不同繁育组合，运用已鉴定的RAPD和SCAR分子标记，分析比较了不同繁育组合子代的DNA扩增情况，探讨了这些分子标记在各个交配子代中的重组分布规律。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector. 3-5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank.

对6例血友病人血清中分离的TTV进行基因克隆，从每例病人的标本随机选多个克隆进行测序，比较其核苷酸和氨基酸序列。

The hereditability of chest diameter is the largest both in half-sib families and clones.N, P and K are three necessary nutritive elements of all plants, which are very important to the growth of plants.

在两片测试林中都以胸径的遗传力为最高，说明胸径在白榆中是遗传性最强的一个性状。N、P、K是一切植物生长所必需的大量营养元素，对植物的生长起着重要的作用。

The hereditability ofmost characters of clones is over 0.8 except for dry weight and transpiration rate.

在后者中，除单叶干重和蒸腾速率遗传力较小外，其它性状都在0.8以上。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

Sequences from 19 randomly selected phage clones indicated that the distribution of nucleotides and amino acids paralleled the expected frequency. The combined index of hydropathy and isoelectric point of these 19 peptides were randomly distributed over wide ranges.

随机挑取了19个克隆，序列分析表明，核苷酸和氨基酸的分布与预期的基本一致。19个多肽的疏水性和等电点的综合指标分布广泛。

After clones landed, the furious Kon'me were further enraged, and opened fire on all off-worlders.

克隆人着陆后，杀红了眼的康美人更加恼羞成怒，不分青红皂白地向所有外来者开火。

After clones landed, the furious Kon'me were further enraged, and opened fire on all off-worlders.

克隆人着陆后，杀红了眼的康梅人更加恼羞成怒，不分青红皂白地向所有外来者开火。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！