查询词典 clones

At the very earliest stage, people too are clones- clones of the fertilized egg .

在最初阶段，人也是克隆体—受精卵的克隆体。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

The results showed that there were intraspecific differences in plant height and stem diameter of tolerant clones, and plant height and stem diameter of sensitive clones decreased significantly.

结果表明：(1)割手密耐性无性系株高和茎径对UV-B辐射响应具有明显的差异；敏感无性系的株高和茎径显著或极显著的下降。

A clone from each genotype was randomly selected as representative for sequencing. The obtained 16S rDNA gene sequences had a similarity of 87%-100% with those in the GenBank (www. ncbi. nlm. nih. gov), and more than half of them had a similarity lower than 97%, being of new species. Based on phylogenetic analysis, the bacteria in the two soils were classified into 10 groups, with 5 groups in common. The dominant bacterial groups in the two soils differed obviously. In primeval forest soil, the dominant group was Proteobacteria, which had 39 genotypes, occupying 58.0% of all the clones; while in the soil of degraded ecosystem the dominant groups were Acidobacteria and Proteobacteria, which had 19 and 15 genotypes occupying 32.5% and 30.5% of all the clones, respectively. In the soil of degraded ecosystem, Proteobacteria group decreased while Acidobacteria group increased markedly, compared with those in primeval forest soil.

从每种基因型中随机选择一个克隆子作为代表进行测序分析，所有序列与GenBank数据库中序列的同源性为87%~100%，且两样地中均有超过一半的基因型序列与数据库中已知序列同源性低于97%，属于分类在"种"地位上的新发现细菌；通过系统发育研究将两样地的细菌分为10大类群，两样地共同拥有5大类群，但两样地的细菌优势类群明显不同，原生土壤为Proteobacteria，含39种基因型，占总克隆子数的58.0%，退化生态系统土壤为Acidobacteria和Proteobacteria，分别含19种和15种基因型，占总克隆子数的32.5%和30.5%；与原生土壤细菌类群相比，退化生态系统土壤Proteobacteria类群明显减少，Acidobacteria类群明显增加。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

77%(35/45) of positive clones from eluted phage clones were obtained by 3 rounds of biopanning.

经过3轮淘洗，获得的阳性克隆率为77%(35/45)。

By the research of 'reselection of high-yield clones of Pinus massoniana', 58 fast-growth and high-yield clones were selected. Their genetic gains were 51.29%(the highest was 116.82%), and could be used to build high-yield seed orchard of Pinus massoniana.

通过"马尾松高产无性系再选择研究"，筛选出58个速生、高产无性系，平均遗传增益达51.29%（最高达116.82%），可用于营建马尾松高产种子园。

In order to clone AVR-Pia, the flanking sequence of MS220K4 was used as a probe to screening the TAC library and 17 positive clones were obtained. Further restriction enzyme mapping and Southern blot analysis on 10 of the 17 positives clones narrowed the avirulence gene to a region of 10 kb.

为克隆AVR-Pia基因，以MS220K4的侧端序列为探针筛选稻瘟菌基因组TAC文库，得到17个阳性克隆，通过对其中10个进行的限制性内切酶分析并结合Southern杂交确定了目的基因所在区域。

Study on genetic variation of seed bearing property at the first clonal Cupressus funebris seed orchard by 75 ramets of 15 clones demonstrated that seed bearing capacity had significant difference among clones, but had high repetition ratio.

利用柏木一代无性系种子园中15个无性系75个分株材料，研究无性系结实性状的遗传变异规律。结果发现，不同无性系的结实能力差异巨大，其无性系重复力较高。

The variation coefficients of most traits are greater in half-sib families than that in clones except for several branch traits.The average height of clones is nearly two meters higher than that of half-sib families, and the average volume of clones is 1.16 times about that of half-brethren families.

除枝下高、侧枝粗、分枝角、冠幅等几个分枝性状在无性系间的变异系数大于在半同胞家系间的变异系数外，其余性状均小于在半同胞家系间的变异系数。20个无性系的总平均树高、胸径和材积高于18个半同胞家系的平均值，其中，树高和材积显然高于半同胞家系的树高和材积，平均树高高出近2米，平均材积高出16.1%。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。