查询词典 clones

Screening of PRL-3-interacting proteins in yeast two-hybrid screening systems Four positive clones in our yeast two-hybrid screening systems were found after Homo sapien fetal brain cDNA Library had been screened.

而用Anti-FLAG M2 Affinity Gel沉淀FLAGPRL-3蛋白的同时，外源蛋白HA-CDH22也一同被沉淀，实验说明外源性的CDH22和PRL-3在体内环境中存在相互作用关系。

Obi-Wan's moralizing encounter with an alien shilling death sticks in "Attack of the Clones" is a bit schoolmarmish but nevertheless true.

欧比王在《克隆人的进攻》中对一个兜售销魂棒毒品的外星人说教，有点老教师般的古板，尽管正确。

There are obvious differences between clones with regard to their self-fertility.

自交能孕性在不同的无性系中是明显不同的。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

Enable it also with any SoundBlaster Pro clones.

对声霸卡Pro相容的也可以用它。

One hundred and eighty-four cDNA clones were identified as submergence specifically induced or highly expressed.

对其中155个cDNA克隆测序并去除重复克隆后，共得到95个差异表达的cDNA片段。

That is almost complete except for the 911 exposure revelations, which will result in a sweep off the board of the remaining ones and all clones and solid-holograms.

那几乎是完全除了 911之外显示被揭露的事，结果将导致大量清除剩余的人和所有和立体全息图克隆。

Only Plo Koon and a trio of clones: Commander Wolffe, Sinker and Boost, survived and were rescued by Anakin Skywalker and Ahsoka Tano.

只有普罗·孔和三个克隆人士兵——沃尔菲指挥官、辛克和布斯特——幸存并被阿纳金·天行者和阿索卡·塔诺营救。

This paper deals with cutting techniques of teak clones based on tube-seedlings.

对柚木组培苗田间扦插扩繁的主要技术开展了研究。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。