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The pALTER plasmid was successfully transfected into CHO cells and positive clones were screened and detected immunohistologically.

结果 含有人CD59两种突变体的重组pLATER质粒经酶切和琼脂糖凝胶电泳鉴定，得到长496 bp的电泳条带，与所插入片段完全相符。

In nature, clones are derived from a single parental organism or cell by mitotic cell division, asexual reproduction, or parthenogenesis.

在自然条件下来源于有丝分裂所产生的单个亲本细胞或生物体，还可能来源于无性繁殖或单性生殖。

The genetic variation of natural stem-join characters of Paulownia fortunei clones was studied by means of field test and statistical analysis.

利用田间试验和统计分析的方法，研究了白花泡桐无性系自然接干性状的遗传变异规律。

This affects every machine with a Pentium processor (not clones, not Pentium Pro or PII), no matter what operating system it's running.

这会影响使用Pentium处理器的所有计算机(不是兼容处理器，不是 Pentium Pro 或 PII)，不论其使用的是什么操作系统。

Then, 10 phage clones were selected from the third biopannirtg with ELISA.

结果显示，噬菌体的产率富集了约200倍。

The results showed that the 20 phagocyte clones, which was selected at random after three times selection, could combine with BmVUB with ELISA analysis.

结果表明，3轮筛选后随机挑取20个噬菌体克隆，ELISA 鉴定表明20个克隆均可与BmVUB结合。

The distribution ratio of photosynthate between root and shoot is another factor that may be contribute to the WUE difference mechanism, for the high WUE clones usually have optimal ratio of root to shoot.

叶片光合作用积累的同化物在地下、地上器官间分配比例的差异也是引起品系间WUE的差异的一个原因，WUE高的品系具有较优的根冠比。

The largest group of clones fell into the so-called TG-1 phylum, which has no cultivated representatives.

这些类群的细菌不均匀地分布于肠道四个不同的微生态环境中。

The major results are as follows:1 250 recombinant positive clones of RAPD microsatellites-enriched library of ramie were rapid screened with PIMA protocol.

本研究采用RAPD富集法与探针杂交富集法首次分离了31个苎麻微卫星序列，建立了以探针杂交富集法分离苎麻微卫星的方法。

Results:Mutant CD59 cDNAs subcloned into the mammalian expression vector PLATER and transfected CHO together with the pcDNA,which confered resistance to G418. The positive clones were tested by FIH.

结果：运用阳离子脂质体导入法将重组pALTER质粒与pcDNA3质粒共转染CHO，成功筛选出的阳性克隆经FIH证明突变CD59可在CHO细胞表达。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。