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In fungal diversity analysis, thirty six of 40 clones were identified as Neocallimastix frontalis and two clones as Aspergillus penicillioides and Pacecilomyces sp., repectively. And the other two clones were recognized as uncultured fungi.

在真菌多样性分析中，40个株系中有36个属於 Neocallimastix frontalis 。，二株各分属於 Aspergillus penicillioides， Paecilomyces sp。，另二株则属於未培养的真菌。

The female parents are 4 choice [1] ness clones in Chinese fir primary orchard. The male parents are 3 polyanthus clones in the gene pool of old Chinese fir, the mixed pollen of these clones and all the clones in this gene pool. The testing woods were founded with 18 cross grouping which were obtained by the using of NCⅡ.

以经过子代测定的杉木初级种子园中的4个优良无性系为母本，老杉木种质资源库中的3个花芽较多的无性系及其混合花粉和种子园混合花粉为父本，采用测交系交配设计进行杂交制种，获得18个杂交组合并以此营建试验林。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

The results showed that there existed variation among eucalypt families in wood physical-mechanical properties and wood chemistry. There were significant differences among E.pellita families、E.grandis families and E.urophylla譋.grandis clones in wood colors as well as between Strains and within trees.The variation of the surface wetability of wood between E.grandis families and E.urophylla譋.grandis clones were significant.Even in the same families level or clones level,there still existed the same regulation. The degree of collapse of eucalypt had a large relation in the amount of tylosis in vessel and vestured pits on the cell wall of vessel. The more rich tylosis and vestured pits in vessels,the more probability to occur collapse for eucalypt.The biggest moment collapse was probably the critical point of the drying degrade.During the fomulation of drying technology,it is necessary to adopt moderate condition to make the vessels which had formed the biggest moment collapse at the biggest moment collapse resume,especially to prevent the permanent set.At the normal temperature level,the main factors which influenced residual collapse were the contents of ray parenchyma and axial parenchyma .Nearly all the cells joined the course of forming the moment collapse and biggest moment collapse.Interval drying was fit for eucalypt plantation which was easier to make collapse.

研究表明：桉树不同家系间木材物理力学性质和化学组成存在差异；粗皮桉家系间、巨桉家系间、尾巨桉无性系间材色存在显著差异，株间、株内木材材色存在变异；桉树木材表面润湿性在巨桉家系间、尾巨桉无性系间存在着显著差异，在同一家系或同一无性系中，株间和株内也存在着相同的变化规律；桉材皱缩程度与其导管中侵填体的多少和导管壁上纹孔具有的附物多少密切相关，侵填体含物越丰富，纹孔附物越多，桉材越容易皱缩；最大瞬间皱缩是能产生更大干燥降等的临界点，在制定干燥工艺时，必须在最大瞬间皱缩发生时，采用温和条件，使已产生最大瞬间皱缩的细胞尽可能多的恢复，尤其不能使其产生永久变定；在常温条件下，影响残余皱缩的主要因子是射线薄壁细胞和轴向薄壁细胞含量；而对于瞬间皱缩和最大瞬间皱缩，几乎所有细胞都参与它们的形成过程；对于易皱缩的桉树木材，间歇干燥是最有前途的干燥方法。

The choiceness varieties of Canarium album were tested in three different places of Guangdong. The study researched the 3-4a trials after engrafting, the results were as follows:(1) The results of variance anaylsis indicated that there were highly significant differences in yield per plant and yield per crown projection area among clones, and the height, stem diameter and crown side were highly significant in some test sites. It showed that genetic diversity existed in yield per plant among clones, and selecting high yield clones were effective.

在高州市大坡镇、古丁镇以及茂名市八一林场三个试点开展橄榄优良品系扩大试验，嫁接后第3年及4年的结实及生长结果如下：（1）方差分析结果，无性系间单株产果量、单位树冠投影面积产果量均达极显著差异水平，树高、径粗和冠幅在有的试点达极显著差异、有的试点无显著差异，表明无性系间结实存在着真实的遗传差异，通过无性系测定选择的高产无性系有效。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。