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The illumination is of no effect on the germination rate of oospores of Phytophthora sojae.30 single-oospore isolates obtained from the test strain of Phytophthora sojae Ps-H2 (Race 1 ) were evaluated using hypocotyl-inoculation method for race phenotype on soybean cultivars. One clone phenotype ( race 1 )was selected randomly for further isolation and virulence identify .

将大豆疫霉菌供试菌株Ps-H_2(生理小种1号)每代挑取30个单卵孢株，进行生理小种鉴定，随机挑选1株1号生理小种的单卵孢株继续进行单卵孢株分离和毒性鉴定，连续两代试验结果表明大豆疫霉菌原始菌株并朱出现毒性分化，表明原始菌株即为1号生理小种的毒性纯系。

The Scorpius Neural Clone personality that was programmed into the neurochip took control of Crichton's body — and forced Crichton to kill his beloved Aeryn.

天蝎的克隆人格被灌输进神经芯片，控制了克莱顿的身体，强迫他杀死他所爱的艾瑞。。。

Kim: Turns out your genius of a client didn't make just one clone for Mr. Haft's World-winning Beckham

原来你的天才当事人不只替 Haft 先生做了只冠军复制狗。

Leigh's wolf-man, Luke, joined all of her forged Scions back into one and shattered it to stop Leigh's unfinished clone, leaving only the pieces for Lara to keep safe at home behind her secret sliding timed door.

李制造出的狼人卢克，把她造出的所有假司祭盎组合在一起打碎，打断了未完成的克隆，而碎片都由劳拉收起，放在她家中一个秘密的限时门之后。

The specific primer was further designed. Using the ray floret as material, after RT-PCR, T-A clone and sequencing, a 732 bp sequence was obtained.

进而设计特异引物，以非洲菊舌状花为材料，通过RT-PCR扩增并经T-A克隆后测序，获得一条长度为732 bp的序列。

Clone of these programs is due to the producers lack of innovation and sole pursuance for watching rate.

克隆现象的成因主要是节目制作方片面追求收视率和缺乏创新思维。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

In order to understand the mechanism of interaction protection whether or not the mechanism induced by dsRNA, we make use of the virus- Tomato Mosaic Virus to construct the inverse overlapped clone through the positive and inverse RNA and intron, which transcriped to dsRNA in outer.

结果发现，与对照相比，当 ToMV 与 dsRNA 一起接种时接种半叶枯斑数明显减少，表明 dsRNA 对 ToMV 有一定的抗性。

One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。