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The illumination is of no effect on the germination rate of oospores of Phytophthora sojae.30 single-oospore isolates obtained from the test strain of Phytophthora sojae Ps-H2 (Race 1 ) were evaluated using hypocotyl-inoculation method for race phenotype on soybean cultivars. One clone phenotype ( race 1 )was selected randomly for further isolation and virulence identify .

将大豆疫霉菌供试菌株Ps-H_2(生理小种1号)每代挑取30个单卵孢株，进行生理小种鉴定，随机挑选1株1号生理小种的单卵孢株继续进行单卵孢株分离和毒性鉴定，连续两代试验结果表明大豆疫霉菌原始菌株并朱出现毒性分化，表明原始菌株即为1号生理小种的毒性纯系。

The Scorpius Neural Clone personality that was programmed into the neurochip took control of Crichton's body — and forced Crichton to kill his beloved Aeryn.

天蝎的克隆人格被灌输进神经芯片，控制了克莱顿的身体，强迫他杀死他所爱的艾瑞。。。

Kim: Turns out your genius of a client didn't make just one clone for Mr. Haft's World-winning Beckham

原来你的天才当事人不只替 Haft 先生做了只冠军复制狗。

Leigh's wolf-man, Luke, joined all of her forged Scions back into one and shattered it to stop Leigh's unfinished clone, leaving only the pieces for Lara to keep safe at home behind her secret sliding timed door.

李制造出的狼人卢克，把她造出的所有假司祭盎组合在一起打碎，打断了未完成的克隆，而碎片都由劳拉收起，放在她家中一个秘密的限时门之后。

The specific primer was further designed. Using the ray floret as material, after RT-PCR, T-A clone and sequencing, a 732 bp sequence was obtained.

进而设计特异引物，以非洲菊舌状花为材料，通过RT-PCR扩增并经T-A克隆后测序，获得一条长度为732 bp的序列。

Clone of these programs is due to the producers lack of innovation and sole pursuance for watching rate.

克隆现象的成因主要是节目制作方片面追求收视率和缺乏创新思维。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

In order to understand the mechanism of interaction protection whether or not the mechanism induced by dsRNA, we make use of the virus- Tomato Mosaic Virus to construct the inverse overlapped clone through the positive and inverse RNA and intron, which transcriped to dsRNA in outer.

结果发现，与对照相比，当 ToMV 与 dsRNA 一起接种时接种半叶枯斑数明显减少，表明 dsRNA 对 ToMV 有一定的抗性。

One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。