查询词典 clone

As the prequels arrived, the imagined Clone War history was given a huge twist.

当前传三部曲上映时，设想的克隆战争历史发生巨大转折。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

We have established two clones which were derived from same piece of emhryogenic multicellular mass of pollen for a vareity Ba Tang Bai.Both Kinds of clone had different differentiation potential in subcultures for over three years.

本实验室筛选出具有不同分化能力的单倍体玉米无性系组织，挑选其大小相同、新鲜的、具有分化能力的无性系No.1和完全丧失分化能力的无性系No.250组织，转接到分化和继代两种培养基上，分析其DNA和RNA以及蛋白质含量的差异，以了解组织在分化过程中DNA、RNA和蛋白质动态。

The protocol is summarized in Figure 18.16. A radioactive probe representing a gene (most often a labeled cDNA clone derived from the mRNA) is hybridized with thedenatured DNA of the polytene chromosomes in situ. Autoradiography identifies the position or positions of the corresponding genes by the superimposition of grains at a particular band or bands.

如图26.15所示为此过程草图：一个放射性探针代表一个基因（常是一个从mRNA中克隆得到的cDNA），它与原位多线染色体的变性DNA杂交，放射自显影仪可通过叠印特定纹带的纹理来识别应答基因的位置。

The protocol is summarized in Figure 18.16. A radioactive probe representing a gene (most often a labeled cDNA clone derived from the mRNA) is hybridized withthe denatured DNA of the polytene chromosomes in situ. Autoradiography identifies the position or positions of the corresponding genes by the superimposition of grains at a particular band or bands.

如图26.15所示为此过程草图：一个放射性探针代表一个基因（常是一个从mRNA中克隆得到的cDNA），它与原位多线染色体的变性DNA杂交，放射自显影仪可通过叠印特定纹带的纹理来识别应答基因的位置。

The clone plasmids of the 3 genes were extracted, then analysed with restriction enzyme and sequenced respectively.

提取克隆菌落的质粒，并进行酶切鉴定和序列测定。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

Also, the fact that he is adventurous, outdoorsy and confident can't hurt. You don't need a clone of yourself to marry.

同理，他喜欢冒险、喜欢室外活动和充满自信都不会造成什么伤害，你不需要嫁给一个克隆的自己。

Objective To clone and express the Tip operon gene of E. coli and increase the enzyme activity of expressed product.

目的 克隆并表达大肠杆菌色氨酸操纵子基因，提高其酶活性。

A different mer operon gene was obtained, which might lay a basis for the further study on the structure analysis and gene clone of it.

获得了缺少merR基因的mer操纵子，具有理论研究意义，并为抗汞工程菌的构建打下基础。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。