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In this research,Young buddings of Clone SCATC 7-33-97 were used as plant materials,adopting to the quadratic general rotary unitized design,with nitrogen,phosphor,potassium,magnesium these four factors with 5 levels(1/2 implement) for fertilizer experiment.

本研究以橡胶幼苗无性系热研7-33-97为试验材料，采用二次回归通用旋转设计安排了氮、磷、钾、镁肥四因素5水平(1/2实施)的肥料试验。

By using the "mechanical wounding"experimental system estabilished by us, and light- and electron microscopy, autoradiography and radioisotope technique, immuno-fluorescence localization, homologous clone and RACE technique, in situ mRNA hybridization and ELISA et al., we investigated the relationship between the transportation of exogenous JA and the secondary laticifer differentiation, the relationship between the producton and accumulation of endogenous JA and the laticifer differentiation from cambia, and the effect of appling exogenous jasmonates on the natural rubber production.

利用我们建立的机械伤害诱导乳管分化的实验系统，采用光学显微镜和电子显微镜技术、同位素示踪和放射自显影技术、免疫荧光定位技术、同源克隆和RACE技术、mRNA原位杂交和ELISA等技术，研究了外源茉莉酸在橡胶树体内的移动及其对维管形成层分化次生乳管的诱导效应、内源茉莉酸与乳管分化的关系以及茉莉酸类物质对天然橡胶产量的影响。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

The repeated vilification of the Neimoidians following the Clone Wars led many of that species to abandon their heritage and pose as Duros.

克隆战争后，很多内莫迪亚人不断遭到诽谤攻击，不得不背门弃宗，伪装成杜罗人。

In the sunlight beautiful noon, in a luxurious villa, General Hawke plays a stringed musical instrument while in teaches own youngest son to sing that sacred song, in this violent storm, the singing sound resounds once more, When the evil soul arrives at the Arab League clone, The serby luo is driving away these dybbuks, Arrived at the hell bog, They soak in here, Felt them once to exert on the good people body's crime, One then has one is starting once again; humph!

在阳光明媚的晌午，一栋豪华的别墅里，霍克将军一边弹琴一边在教自己的幼子唱着那神圣的曲子，在这狂风暴雨中，歌声再次响起，当罪恶的灵魂来到阿克隆，塞比猡驱赶着这些恶灵，来到了地狱的沼泽，他们在这里浸泡，感受他们曾施加在善良人们身上的罪行，一便有一便的周而复始着

NSCs existed in the occipital of cortex of different fetal age included middling round cells, NSCs had enations from 0 to 1. Nucli were larger than plasm. Each NSC had nucleoli from 2-4 and rarefaction chromatin. Most of NSCs distributed in three growth modes including crowd, cluster and clone, occasionally with a single growth mode among other nerve ceils.

NSCs呈明显的区域性分布，大部分NSCs以群状、簇状或数个细胞形成的小集落样生长方式存在，少数NSCs以单个形式存在于其他神经细胞中，各胎龄组NSCs在分布、形态、种类、生长方式等方面无明显差异。

Objective: To clone and express human MAGE-C2 gene and to investigate the expression pattern in transfected eukaryote cells.

目的：克隆人黑色素瘤相关抗原MAGE—C2的cDNA，构建真核、原核表达载体并转入相应细胞获得表达。

Extracting exonic sequences directly from genomic sequence is difficult, but ESTs have provided a convenient means of accessing them. An expressed sequence tag is part of a gene that results from sequencing a portion of a cDNA clone that was generated from an mRNA.

从mRNA来源的cDNA文库中随机取出一个克隆，从5'末端或3'末端对插入的cDNA片段进行单向测序，所获得EST为我们研究基因表达提供了方便快捷的手段。

Objective To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China.

目的克隆并分析华南地区粉尘螨主要变应原Derf2的cDNA片段。

We used the NIH3T3 fibroblast cells ascarrier because the cell clone is easy to expand,to betransfected and to express the exogenous gene.In the presentstudy,a model of fibro blast cell-mediated human IFN-〓 genetherapy was established and its anti-tumor activity was observed.

为此，我们选择了易于扩增、易于转染并能稳定表达外源目的基因的成纤维细胞为载体细胞，建立了人〓基因疗法的实验模型，并观察了其体内抗肿瘤作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐