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At the same time, based on the extracted total RNA from the leaves of Zostera marina L., the cDNA clone could be obtained and the Na+/H+ antiporter gene could be studied by applying the techniques of RT-PCR and RACE. Its expression profile could be studied when applying the Northern technique.

同时在所成功提取出的大叶藻叶片组织的总RNA基础上，做出cDNA克隆，再通过RT-PCR和RACE技术等研究方法，来试图探究大叶藻的Na~+/H~+逆向转运蛋白基因，然后可结合Northern技术来研究其表达情况。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

Clone 667 is characterised as rough and possessing black fruit and gamey notes .

作为拥有777强的特点是克隆黑色水果。

Clone 667 is characterised as rough and possessing black fruit and gamey notes.

克隆是形容667黑粗、藏水果腥味笔记。

In the middle of last century,biologists put a somatic cell nucleus of a frog into an enucleated egg cell,then created a clone tadpole.

在上个世纪中期，生物学家把青蛙的体细胞核放入青蛙的去核卵细胞里，结果制造出了克隆蝌蚪。

These OMPs were expressed and purified using molecule clone methods and polyclonal antisera were gamed by immunizing rabbits separately.

于是，我们应用免疫学方法从体液免疫和细胞免疫水平分析这3个外膜蛋白提高机体的免疫力的机制。

Jango handpicks her to assist in the training of the clone commandos, where she hammers their independent natures into razor-edged power.

詹高。费特挑选她协助训练克隆突击队，将他们从独立个体锤炼为锋利的剑刃。

Methods Three pairs of primers have been designed according to the hilA of Salmonella spp., hemolysin of Proteus mirabilis and Staphylococcus aureus clone pSa-442 Sau3AI fragment and the anticipated PCR products were 580bp, 401bp and 256bp.

本研究依据沙门菌侵袭基因正调节蛋白基因、变形杆菌溶血素基因和金黄色葡萄球菌特异性pSa-442序列，运用Primer Premier 5.0分别设计3对特异性引物，预计PCR扩增的目的基因片段分别为为580bp、401 bp、256 bp。

A clone is said to be positive if it hybridizes with the probe, and negative otherwise.

克隆如果含有这个特定因子则称它为正的克隆；反之，称为负的克隆。

Find a YAC or BAC clone to which the marker probe hybridizes

。寻找可与标记探针杂交的 YAC 或 BAC 文库克隆

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。