查询词典 clone

Objective To clone and sequence variant-specific surface antigen gene from Giardia lamblia isolate SUCH/89/BTMRI/2（C2） derived from human in China. Methods Total genomic DNA of G.

摘要目的克隆并测定中国人源蓝氏贾第鞭毛虫滋养体SUCH/89/BTMRI/2（C2）的表面变异抗原基因序列。

Avian leukosis virus ; subgroup J ; env gene ; clone ; expression

禽白血病病毒； J亚群； env基因；克隆；表达

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后，取脾细胞和SP2/0骨髓瘤细胞进行融合后，以间接ELISA和有限稀释法进行筛选，获得4株能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞株，该株单克隆抗体命名为1F2，2C2，3D6，4H6株。

We employed an easily observable and manipulatable system in which activated and non-activated T-cells were co-cultured with a stable well-characterized clone of lacZ-expressing murine NSCs.

我们采用了直观且简单易行的系统，将活化和未活化的T细胞与稳定的lacZ表达的鼠类NSCs共培养。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

Chieftain with the degenerate primers by RT-PCR.The cDNA clone of 3GT encodes a predicted polypeptides of 448 amino acids,and the deduce protein showed 76% highest identity with 3GT of Petunia hybrida and Solanum melongena,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a new member of the corresponding 3GT-family enzymes.

序列分析表明，这个3GT基因编码的多肽为448个氨基酸残基，在氨基酸水平上与茄科的矮牵牛和茄子的一致性最高，均为76%，多重比较和系统发育分析均表明，该基因为3GT家族中的一个新成员。

Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

In measurements of a day,generallyspeaking,the photosynthesis of birch in mesophytic habitat is better than that in xerophytichabitat(peak values are 12.8,10.33μmolCO2m-2s-1 respectively);that of sexual birch inmesophytic habitat is better than that of clone birch(peak values are 9.87,6.71μmolCO2m-2s-1respectively);that of young tree is better than that of seedling(peak values are12.37,10.05μmolCO2m-2s-1 respectively).

在一天中的各个时刻，总体说来，中生生境生长的白桦光合作用超过旱生生境生长的白桦光合作用(净光合速率峰值分别为12.8、10.33μmolCO2m-2s-1)；白桦幼树的光合作用超过白桦幼苗(净光合速率峰值分别为12.37、10.05μmolCO2m-2s-1)；中生生境有性白桦的光合作用超过无性白桦的光合作用(净光合速率峰值分别为9.87μmolCO2m-2s-1、6.71μmolCO2m-2s-1)。

Man takes the reins of the most delicate tiny mysteries of microcosmos by means of anatomize gene and clone life.

剖析基因，克隆生命，人类掌握微观世界最微妙的奥秘。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。