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The distribution of PTA1 molecule on NK cell, NK cell lines and NK cell clone was detected by indirected immunofluorescence staining and FCM analysis. The results showed that PTA1 expression rate on activated NK cell in MLC was above 60%.

结果证明这种方法能够稳定获得NK细胞克隆，应用流式细胞仪检测获得的一株NK细胞克隆表型为 CD56CD3－。

We construct pCDNA3.1-TfRexpression plasmid successfully, there are higher expression of TfR aftertransient and stable transfection; we get positive cell clone of containingpCDNA3.1-TfR, establish infarctate foundation to empirical study ofmolecular imaging of MR.

成功构建了pCDNA3.1-TfR表达质粒；质粒转染U251细胞后瞬时与稳定表达的TfR蛋白明显增高；成功筛选出pCDNA3.1-TfR阳性细胞克隆，为分子影像学的实验研究奠定了坚实的基础。

In order to reach this goal,the primary basic research work is to clone,evaluate and analyse the function of the genes that support or impact the development of malaria parasites in the mosquito vecter and the study on the mechanisms of innative immune defence responses against Plasmodium infection.

于上世纪九十年代，WHO提出了基因操纵蚊媒的新策略，而寻找支持或抑制疟原虫在蚊体内发育的相关基因，克隆、鉴定及分析其功能，以及研究按蚊抗疟原虫感染的先天性免疫反应机制是实现这一策略的重要前提。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变婴儿检测系统中，通过一些不表达的中性靶基因所婴儿检测到的突变规律肿瘤是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变检测系统中，通过一些不表达的中性靶基因所检测到的突变规律是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起流行的稻叶1d为同 RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以前四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起大流行的稻叶1d为同一RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

To clone the calmodulin genes from Karelinia caspica, Kalidium foliadum and Betula halophila in Xinjiang, designed primers were used to amplify 450-bp cDNA fragments with RT-PCR.

提要 采用RT-PCR扩增的方法，从新疆盐生植物花花柴、盐爪爪和盐桦中分别克隆获得了450 bp的cDNA片段。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

Objective To clone and sequence variant-specific surface antigen gene from Giardia lamblia isolate SUCH/89/BTMRI/2(C2) derived from human in China.

目的 克隆并测定中国人源蓝氏贾第鞭毛虫滋养体SUCH/89/BTMRI/2(C2)的表面变异抗原基因序列。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。