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In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

Objective Chronic myeloid leukemia, with of chromosome, is one of primary clonal malignancies of hemapoietic stem cell. Philadelphia chromosome is the hallmark of malignancies clone in CML.

研究背景与目的：慢性髓系白血病是一种原发于多能造血干细胞的恶性克隆性疾病，第9号与第22号染色体相互易位形成Ph染色体是其肿瘤恶性克隆性标志。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

Findings in clinical research show that life regenerative substances can activate and culture the healthy organ cells to regenerate and clone, while at the same time, these life regenerative substances can prevent the normal cells from canceration and can induce apoptosis of the cancerous tumor cells. Based on the animal experiments, we have successfully produced the nutritious foods with life regenerative substances according to the national food safety standards. Since Jan 25th 2008, more than 300 cases of cancer patients who had given up the traditional medical treatment accepted our holozoic diet therapy with the life regenerative substances, and remarkable regenerative effects have been obtained. The potential regenerative ability of the organ cells are actived by the life regenerative nutrients, thus the cancer patients continue to struggle against with cancers.

我们研究发现，人体器官的再生物质，在促进正常细胞再生克隆的同时，能阻止细胞被诱导成癌细胞，能使癌细胞凋亡性死亡，从而在进行了动物实验的基础上，将这些再生营养物质制作成符合国家标准的营养食品，于2008年1月25日开始进行了300多例放弃医学治疗的临终癌症病人的全营养食疗养生，获得了再生复原的效果，养生的病人仍能通过再生营养的作用，调动人体的再生潜能，继续与癌症病魔作斗争。

To screen the sensitive and resistant yeast mutant when they treated with drug, the recombine technology of yeast gene should be applied to obtain the clone expressing the homeotic gene.

通过使用药物对特定的酵母基因突变株进行干预，筛选出对药物敏感或抵抗的突变株，然后利用酵母基因重组技术，使突变株再表达同源的基因，从而获得再表达同一基因的克隆。

The sequence of amino acid of clone CR271 was highly homologic (61% identify and 76% similarity) to blight resistance gene Xal of rice to Xanthomonas oryzae pv. Oryzae. All other fragments showed significantly amino acidhomology to the NBS-LRR type resistance gene that have been cloned. The length of JR2 and JR3 fragments were 1321bp and 896bp respectively, which did not showed significantly homology on the level of amino acid and nucleotide acid to the sequences that have been known.

其中CR271与水稻白叶枯病抗病基因Xa1(AB002266)氨基酸同源性有61％相同，76％相似，其它片段与已克隆的NBS-LRR类抗病基因同源片段都具有较高的同源性；所获得的右翼序列JR2DNA片段长为1321bp，JR3DNA片段长为896bp，此两片段在核苷酸水平及氨基酸水平上均未找到有显著同源的序列。

Lucidum. Then we clone the gene, glfks1 and glfks2. Hydropathy analysis predicts that GlFks1 and GlFks2 are integral membrane protein with about 16 and 14 transmembrane helices.In genomic southern blot analysis, glfks1 and glfks2 both are single copy gene.

cerevisiae相关之β-(1，3)-D-葡聚醣合成酶基因fks相似之序列，并将其选殖出来，分别得到glfks1与glfks2，利用hydropathy分析，显示GlFks1与GlFks2分别具有16个与14个transmembrane helix，且推测GlFks1与GlFks2应也是属於嵌入的membrane protein。

Theoretically, its calculation complexity is studied, while it is tested empirically by learning multi-samples generated randomly. The comparative analysis and applications show it has some merits of rapid searching and good clustering effect. B3. An immune network optimization algorithm with information feedback, which can solve special complex function optimization problems with high dimensions which objective functions are formed by summing multi-subobjective ones, is constructed based on the clone selection principle and the immune network regulation of the idiotype immune network.

为了对免疫系统中抗体如何学习抗原特征的整体行为有一定的认识，从单个抗体识别抗原及多个抗体并行识别抗原的特征出发，利用免疫应答的相关原理及免疫细胞的免疫功能设计了适合于批量样本聚类的抗原特征学习算法，理论上分析了其计算复杂度，获得了此算法中的关键参数与其搜索性能的关系；应用上，该算法通过对实际的多样本学习，获得了搜索速度快及聚类效果好的优点，比较分析表明这种方法是可行的和有效的。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

Methods: Clone the genes of ScFv (OC183B2) and PE38 into PET30a and then express the immunotoxin after induced with IPTG.

采用基因工程技术，经过3次亚克隆，将编码单链抗体的基因及编码毒素的基因亚克隆至原核表达载体pET30a上，并在IPTG的诱导下进行表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。