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Objective To clone and express thanatin mutant in Escherichia coli and purify it.

目的：在大肠杆菌中克隆表达thanatin突变体蛋白并纯化。

With the outbreak of the Clone Wars, though, Jukassa was never able to hone his farseeing abilities.

当克隆战争爆发时，祖卡萨仍然没能很好地掌握预见能力。

On the basis of investigation and measurement by sampling of the alone tuft in the blooming stage, characters of growth and production on the clone of Festuca arundinacea in middlemountain conditions in subtropical region of China were quantitatively analysed in this paper.

根据以分蘖丛为单位的调查与测定，定量分析了亚热带1800m中山立地条件下苇状羊茅无性系的生长与生产特征。

Objective To clone the flagellar biosynthesis genes fihA, flhB2 and fliR of Leptospira interrogans and construct their prokaryotic expression system Methods Extract genomic DNA from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain 56601 by phenol-chloroform method and amplify fihA, flhB2 and fliR gene fragments by high fidelity PCR.

目的 克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR，并构建其原核表达载体。

In the same observation time, the number of maternal ramets and daughter ramets, the total length and the number of stolon and branching intensity of P. flagellaria clone population were higher than P. anserina. But the average length of spacer and stolon of P. anserina were more than P. flagellaria.

在同一观测时期内，单位面积内蔓委陵菜无性系种群的母株数量、分株数量、匍匐茎总长度、匍匐茎条数以及分枝强度均高于鹅绒委陵菜种群；而鹅绒委陵菜的间隔子平均长度、匍匐茎平均长度均大于蔓委陵菜。

Objective: To clone the 5' flanking region of the human CD34 gene containing transcriptional regulatory sequence.

目的：克隆有特异调控活性的人CD34基因5'端转录调控序列。

In the condition of suspending culture, the gametophytic clone has much higher instantaneous growth rate than stilling culture.

在充气悬浮培养条件下克隆的瞬时生长率明显高于静置培养。

60Co-γray was a useful mutagen in induced mutation breeding .After treated with 60Co-γray ,free-living conchocelis of wild Porphyra haitanensis were induced and represented in offsprings. Purple mutant and excellent practical characters strains were selected from a lot of gametophytic blades. Single cell clone and enzymolysis were used to propagate fast in pure strains of induced mutations.

坛紫菜野生丝状体经一定剂量60Co-γ射线辐照后，子代叶状体发生了变异，经大量培养后筛选出紫色突变体（3号Ⅰ）和经济性状优良的个体（7号Ⅰ和7号Ⅱ），通过体细胞酶解和单克隆技术快速获得诱变选育纯系，并对其主要经济性状进行了研究。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp，与预期设计的大小一致，该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2％，但所含的启动子序列元件的数量和距离上很相似，均具有有典型的TATA盒和CAAT盒，以及种子特异表达所必须的调控元件。

A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L.

利用PCR筛选方法从陆地棉纤维cDNA文库中筛选到一个全长cDNA序列，命名为GhbZIP。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。