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It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因，在昆虫杆状病毒表达系统中进行了表达，并对表达产物进行了纯化，为深入研究BiVP的生物活性奠定了基础。

Objective To clone the Bombus ignitus venom protease gene screened from the expression sequence tags of the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell.

目的 克隆表达序列标签中筛选出的红光熊蜂蜂毒蛋白酶基因，在草地夜蛾细胞Sf-9中进行表达。

For example,the height,the diameter at breastheight and the volume of B301 of 8 years were 39%,72% and 255% largerrespectively than those of the contrast of YiXian Female clone of P.tomentosa.

如此之高的生长量，甚至已接近速生黑杨的水平，具有十分可观的增产效益。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

Osteoblast clone cultivated by adult rat calvaria as culture model in vitro, which can be used to screen new anti-osteoporosis drugs with promoting osteogenic action.

本研究从成年SD大鼠颅骨分离培养成骨细胞，方法简便、快速，是筛选促进成骨细胞骨形成的抗骨质疏松新药的良好体外模型。

One such cell, led By Soontir Fel's clone Carib Devist, defected to the Republic.

其中一个由苏提尔克隆体加里布·德维斯特领导的克隆人投奔了共和国。

Objective To clone, express and characterize the panallergen profilin from the pollen of Caryota mitis Lour.

目的 克隆并表达短穗鱼尾葵花粉中泛变应原肌动蛋白抑制蛋白。

AIM : To clone the gene of collagen type Ⅰ, to study its expression in lens epithelium of normal and age-related cataractous people, and to compare the difference of their expression.

目的：克隆Ⅰ型胶原基因并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞的表达，并比较二者表达的差异。

AIM : To clone the gene of collagen type Ⅰ, to study its expression in lens epithelium of normal and agerelated cataractous people, and to compare the difference of their expression.

Z！qiz\+qA+VV0 目的：克隆Ⅰ型胶原基因并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞的表达，并比较二者表达的差异。

In this study, 526 base pair DNA fragment of OMT were obtained from Chamaecyparis formosensis. Then the 5' and 3' RACE coupled with the Genome walking were used to clone the full length of CCoAOMT, named CfCCoAOMT. After combining the sequence data, there is a total of 1721 bps in full length including promoter sequence.

本研究於红桧试材中，选殖出526个碱基对之Caffeoyl CoA 3-O-methyltransferase片段基因序列，再经由5'和3' RACE及Genome walking，共获得计有1721个碱基对，包含启动子及基因全长，命名为CfCCoAOMT。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。