查询词典 clone

In order to prevent linear PCV2 cyclization, PCR mutagenesis was used to construct the first molecular clone (pSK-2PCV2) by ligating two copies of the complete PCV2 genome with the pBluescript SK vector. In addition, pSK-PCV2 and ds-PCV2 were constructed. PK-15 cells were transfected with above three infectious clones.

本研究应用PCR诱变技术解决了外源片段易于自连的难题，成功将2个PCV2 SD1株全基因组(DQ346683)头尾相接插入到真核生物表达载体pSK的多克隆位点中，构建重组质粒pSK-2PCV2；另外课题组成功构建含单个PCV2全基因组的pSK-PCV2和自身环化质粒ds-PCV2。

Oftware Description: About Star Defender, This vertical scrolling space shooter is an advanced clone of Space Invaders.

tar Defender 这款垂直滚动太空射击游戏是太空入侵者游戏的高级克隆版本游戏。

In viticulture, a clone refers to a vine variety that is selected for specific qualities which result from natural mutations.

在葡萄栽培领域，一株单体指的是由于自然的突变，而产生的具有特殊品质的葡萄品种。

She was later discovered by clone troopers Waxer and Boil during their recon of the village.

随后，两名克隆人士兵"蜡匠"和"沸腾"在村里侦察时发现了她。

This paper presents the seed origin selection and clone selection of white poplar in Tianshui district of Gansu province, which include 8 seed sources and 220 clones.

本文介绍了毛白杨8个种源， 220个无性系，在甘肃天水地区开展的种源选择试验及优良无性系筛选结果。

Reverse Transcription-Polymerase Chain Reaction was used to clone the whole-length gene of BMP-2 and construct AdV vector.

应用反转录-聚合酶链反应法克隆出骨形态发生蛋白质类2全长基因，构建重组腺病毒载体，DNA-磷酸钙共沉法将辅助质粒PJM17转染293细胞，同源重组构建出重组腺病毒。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

A mutation type and a wideness type were established by clone and gene sequence detection.

基因测序技术对无缺失片段进行克隆、测序，筛选出突变型及野生型。

You'd have to greatly multiply the workweek and clone the teachers.

要想真的这么做，你就得延长工作时间或增加教师的数量。

Snake:蛇: Snake 蛇 is a clone of popular Snake or Worm game.

克隆是一个流行的蛇或蠕虫的比赛。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。