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Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Then the recombinant plasmid transformed E.coli.JM109. One positive clone was obtained from the libraries with casein flat and ELISA sieve method.

用酪蛋白平板法和酶联免疫吸附的活性筛选方法从基因文库中筛选到一株产海洋低温碱性酶的阳性克隆（命名为pHH1）。

Since the establishment of the company, we have adhered to the management concept of "Take quality as the foremost, regard people as the soul". With the advanced imported equipment, well-chosen material, professional production management and sincere after-sales service, we have grown rapidly in the competitive market and developed into a key enterprise with an annual output of over one million square meters in laminated floor production. Our products now include flooring of crystal surface, macro-embossed surface, mirror surface, solid wood, clone solid wood as well as the new generation of light catalyst antibiotic series, among which are feature silk series, tree bark-like series, water-resistant series and our latest innovation, the fantasy series. Over 100 varieties are available. Our products have already been exported to the United States, Canada and some European countries. In China,"Shan Shan Lubao" is becoming a well-known brand among the consumers.

本公司是一家专业生产高档强化复合地板厂家，自公司成立伊始，一直秉持"以质为本，以人为魂"的经营理念，依靠先进的进口设备，精选的优等材料，专业的生产管理和完善的售后服务在竞争激烈的市场里脱颖而出，在短短几年中迅速发展成为一家年产强化地板百余万平方的国力强化地板生产骨干企业：目前公司产品已拥有水晶面、浮雕面、镜面、赛实木、克隆实木、新一代的光触媒抗菌羽丝面系列、光触媒抗菌树皮纹系列、光触媒抗菌防水系列、光触媒抗菌静音系列和最新科技创新产品、光触媒抗菌梦幻系列等，百余种花色，产品远销美国、加拿大、欧洲等国家，在国内，"杉杉绿宝"亦逐渐成为业内及消费者心目当中较知名的品牌。

To clone human sodium/iodide symporter genes, and study the function expression of hNIS in non-thyroid tumor cell.

克隆人的钠／碘同向转运体基因可编码序列，并研究其在非甲状腺肿瘤细胞内的功能表达。

Objective To clone human mucin 1 （MUC1） gene promoter and apply to drive human sodium/iodide symporter gene targeting expression in pancreatic carcinoma cells.

目的克隆人Mucin1（MUC1）黏蛋白的启动子序列，并研究其驱动人钠／碘共转运体基因在胰腺癌细胞内的靶向性表达功能。

Her clone troopers identified her as a traitor to the Republic and blasted her speeder.

她的克隆人士兵将她视为共和国的叛徒，向她的摩托开火。

RESULTS: Some cell colonies were detected at 4 days after pDC316-SOX-9 plasmid transfection. Spindle-shaped cells were collected after clone amplification.

结果：pDC316-SOX-9质粒转染4 d出现小片的细胞集落，挑取克隆扩增培养后，细胞呈梭形纤维状。

The results showed that the techniques could increase gametophyte adhesion rate by over 80%, made the seedlings stronger and the germination quality reach state standard of Class Ⅰ. The growth speed of clone adhesion (8~16 rows) was about 20 days faster than that of normal sporophyte seedling adhesion.

结果显示，这样的措施可使配子体附着率达到80%以上、幼苗健壮、出苗质量达到国家一类帘标准，使克隆采苗自8～16列c期间的生长发育速度较常规孢子体采苗后的生长发育速度快20 d。

Intrigued by this Japanese connection, Star Wars: Clone Wars Art Director Paul Rudish turned to the Land of the Rising Sun as inspiration for Ventress' vehicle.

星球大战：克隆人战争》的艺术导演Paul Rudish受这个日本文化思路的启发，从其中发展出文崔斯战机的设计灵感。

But Stunner was knocked out and Doc Ock was murdered by the insane Peter Parker clone named Kaine .

但是尤物遭淘汰和文件玉珠被谋杀的疯狂彼得帕克克隆命名凯恩。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。