查询词典 clone

And ruthlessly clone each and every step as you ascend the ladder to your own long-overdue freedom and success.

克隆和无情的每一个步骤，你登上梯子到您自己早就应该自由和成功。

Methods Silence the expression of RhoC gene in Bel7402 cells by RNAi. Determine the levels of apoptosis gene and apoptosis-related gene by FACS and RT-PCR, and the migration and growth of cells by scarification healing test and soft agar clone formation test.

以RNAi沉默Bel7402细胞RhoC基因的表达，FACS和RT-PCR检测细胞凋亡和凋亡相关基因水平，细胞划痕损伤和软琼脂克隆形成试验检测细胞迁移和生长特性。

Methods: Soft agar clone forming test, cell morphology, scratch test, MIT and flow cytometry were used to examine the changes of Hep-2 cells treated by different concentration of PB in vitro. Results: There was a dose-dependent and time-dependent inhibition of the cell proliferation by PB.

应用细胞生长曲线测定法、软琼脂克隆形成试验、细胞形态观察、划痕试验、MTT法、流式细胞术等对在体外经不同浓度PB处理过的Hep-2细胞进行检测，观察细胞分化程度及运动能力的改变。

In this study, homologous amplification and RACE technique were used to clone the encoding gene (SpTPS1) of trehalose-6-phosphate synthase from Selaginella pulvinata, which is an endemic exrophilous plant in China. The full-length cDNA is 3223 bp long, containing an open reading frame of 2790 bp.

本研究以我国特有旱生植物垫状巻柏为材料，采用同源扩增与RACE技术相结合的方法克隆了海藻糖-6-磷酸合成酶基因SpTPS1，cDNA全长3223 bp，包括一个2790 bp的开放阅读框。

This became the de facto color for all things Senatorial or Coruscant executive during the Clone Wars.

这事实上成为克隆人战争期间所有属于参议院或科洛桑的东西的颜色。

If his serape was removed, he would be revealed to be wearing clone armor!

如果脱掉他那件披风，你会发现他穿的是克隆人的盔甲！

The insignias on the helmet, shoulder, and serape appear new -- did you pull these from existing source material or were they devised specifically for The Clone Wars?

头盔、肩甲以及披肩上的纹章似乎是新出现的——你是从现存原始资料里找出来的，还是专为《克隆人战争》设计的？

The sequencing result shows that this sequence is 1978 bp at full length. This means that the specific fragment could be used as a base to clone sex-linked gene of Spinacia oleracea L.

测序结果显示，该片段全长1 978 bp，该特异条带的获得为菠菜性别相关基因的克隆奠定了基础。

This study was designed to clone the duck FABP gene based on the sequences of chicken and mammal FABP gene by comparative genome and bioinformatics approaches; to investigate the expression characterization of FABP genes by RT-PCR; to detect the polymorphisms of FABP genes by DNA sequencing, PCR-SSCP methods, and to investigate the effects of FABP genes polymorphisms on growth and body composition traits in Kunshan sheldrake, Cherry Valley Meat duck and so on.

本研究以鸡、哺乳动物FABP基因序列为基础，通过比较基因组学和生物信息学等方法克隆鸭的FABP基因；采用RT-PCR方法研究FABP基因的组织表达特性。

Objective To observe the influence of gene transfection of inducible nitric oxide synthase to the transplanted human laryngeal squamous carcinoma in nude mice, to investigate the expression and the correlayion of iNOS and p53, and to proke into the possible apoptosis mechanism. Methods Transfect the gene of iNOS to Hep-2 human laryngeal cancer cell, sieve out positive clone cell and amplify.

目的 观察诱生型一氧化氮合酶(inducible nitric oxide synthase， iNOS)基因转染对人喉鳞癌细胞裸鼠移植瘤生长的影响，研究在体人喉鳞癌细胞中iNOS与突变型p53基因的表达，探讨二者的相关性及促进肿瘤细胞凋亡的机制。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。