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Objective To clone human ornithine decarboxylase antizyme mutation gene and express its recombinant protein.

目的 构建人鸟氨酸脱羧酶抗酶突变基因，重组至原核表达载体中并表达出重组蛋白。

The coding sequences of Igf2 have been reported in many mammalians, a few birds, amphibian and osteichthyes. But it has not been reported in goldfish. In our investigation, goldfish's insulin-like growth factor Ⅱ gene clone was obtained by PCR.

在许多哺乳类，少数的鸟类、两栖类和硬骨鱼类中，胰岛素样生长因子2的编码序列已经被确定，但是金鱼的Igf2尚未见报道。

One positive clone that can integrate specifically with ouabain with higher affnity was sequenced.

选取一个与特异性抗原结合活性比较高的噬菌体抗体阳性克隆，将其V区基因克隆后进行序列分析。

The search results of two clones ovular differential proteins showed that some of factors that peroxidation and bio-molecule damnification hurt frame and function of cell and producing ethane because sterile clone ovule was devoid of protected enzyme system.

由于胚珠较可育系存在较少量糖酵解必需的酶，加之碳源短缺可能激发糖原异生过程，使胚珠生长发育所需中间产物减少及能量短缺。

FISH with the tomato 45S rDNA clone revealed very strong signal in the satellite on the short arm of chromosome 2 as well as weak signals in five CPD banded regions at pachytene or four pairs of CPD banded regions at metaphase.

用番茄的一个45S rDNA 克隆进行的荧光原位杂交，不仅在位于2号染色体短臂的随体上显示了强的杂交信号，而且在粗线期染色体的5个CPD带区或有丝分裂中期染色体的4对CPD带区显示了弱的杂交信号。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒，并与pCDNA3质粒，按3：1比例混合后，运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO)，用新霉素类似物G418初步筛选出阳性克隆。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

For the first time, this paper presented the concepts about two basic deformations of Pascal's triangle, that is, Shifted Deformation and Clone Deformation.

本文首次提出了杨辉三角的两种基本变体的概念，即错位变体和克隆变体。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA，脂质体siNEO FX转染Hela细胞，分别用MTT法，双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢，软琼脂克隆形成率降低，G0/G1，期细胞比率增加。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。