查询词典 cloak-and-dagger

The special features of this plan are its interdisciplinary (anthropological, historical, and literary) combination of methods and visions and its broad use of artifacts, documents (investigative reports, local gazettes, travel writings, and even novels and plays), and visual images in presenting and expounding upon the elements of "people and locality"(people and their relationship with their environmental ecology),"people and people"(the various social identities and distinctions between peoples), and "people and heaven"(the life customs and religious beliefs of humankind) associated with these archived items.

本计划之特色在於结合跨学科方法与视野，广泛运用各种文物、文书（调查报告、方志游记、乃至小说、戏曲等材料）与图像资料，来诠释与呈现这些材料背后的「人与地」、「人与人」(人群间各种社会认同、区分)、「人与天」（人类之生命礼俗与宗教信仰）等情境，同时呈现中国族群研究的多元论述与探讨中国民族形成的历史过程。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

But when we see a lot of framed timbers, different portions of which we know have been gotten out at different times and places and by different workmen,--Stephen, Franklin, Roger, and James, for instance,--and when we see these timbers joined together, and see they exactly make the frame of a house or a mill, all the tenons and mortices exactly fitting, and all the lengths and proportions of the different pieces exactly adapted to their respective places, and not a piece too many or too few, not omitting even scaffolding--or, if a single piece be lacking, we see the place in the frame exactly fitted and prepared to yet bring such piece in--in such a case we find it impossible not to believe that Stephen and Franklin and Roger and James all understood one another from the beginning, and all worked upon a common plan or draft drawn up before the first blow was struck....

然而，当我们看到这许多木料组成的构架，它们不同的部件在不同的时间和地点由不同的工匠所造出──斯蒂芬、弗兰克林、罗杰和詹姆斯等等──当我们看到这些木料拼在一起，看到它们天衣无缝地组成了一座房子或一间工作坊的构架，所有的掉头和榫眼密切结合，一切构件的长度和大小都使其精确地各归其位，一块也不多，一块也不少，甚至也没忘了搭置脚手架──或者说如果构件少了一块的话，构架上也留下了一处空位来补其所缺──在这种情况下，我们觉得不可能不相信斯蒂芬、弗兰克林、罗杰和詹姆斯从一开头就心照不宣，共同制定出一个计划或方案，然后打出第一拳

Two series of polyurethane acrylate grafted acrylic resins (Acr-g-TPU-PETA and Acr-gPPU-PETA) self-emulsifying systems are obtained by grafting -NCO group modified polyurethane acrylates with acrylic copolymer prepared from n-butyl acrylate, styrene,βhydroxyethyl methacrylate and acrylic acid The self-emulsifying systems showed Newtonian fluidity with viscosity closed to that of water, the solid contents were around 30% The T〓 of 15～4℃ for dried film before photocuring favored film formation at room temperature DSC and DMA results showed that PU-PETA is compatible with acrylic resin in the grafted resin The TG and dTG curves for Acr-g-PU-PETA samples were substantially different from the corresponding blends, and the brodened dTG peaks for photocured grafted resin suggest that there might be multiple interactions among chain segments The photocued film of the grafted resins self-emulsifying system exhibited good overall properties with polyurethane acrylate grafts ranging from 30% to 50%, and Acr-g-TPU-PETA series showed better properties than Acr-gPPU-PETA series The adhesion of the photocured film was 1～2 grade, impact strength was 50kg-cm and flexibility was 1mm The Shore hardness and the adhesion of the film decreased markedly if PETA was replaced by HEMA as photoactive group due to the lower C=C double bond content in the grafted resin The polyhydroxy amine-linked thioxanthones photoinitiators can be evenly dispersed in self-emulsifying system without color spot appearing in the dried film The films photocured with IV〓～IV〓 generally exhibited higher Shore hardness, gloss and solventresistance than that cured with Irgacure 2959 and did not induce the rusting of the iron substrate Pigmenting with titanium oxide had no effect on the photocuring The polyhydroxy amine-linked thioxanthone IV〓 and IV〓 can be new candidates for the formulation of titanium oxide pigmented photocurable water-borne coatings for their pale-yellow appearance and slight interference with the color of the coatings WB 4759 with high water-solubility coalesced and separated from the dried resin film, and even induced the rusting of iron substrate.

以丙烯酸丁酯，甲基丙烯酸-β-羟乙酯，丙烯酸及苯乙烯合成厂丙烯酸共聚树脂，并与含-NCO端基的聚氨酯丙烯酸酯接枝反应，经水性化后，得到两个系列（Acr-g-TPU-PETA和Acr-g-PPU-PETA）各5个不同配比的丙烯酸树脂接枝聚氨酯丙烯酸酯自乳化体系，粘度与水相近，为牛顿流体，固含量30％左右。光固化前干燥涂膜的玻璃化转变温度在-15℃～4℃之间，有利于室温下成膜。DSC及DMA谱表明两种树脂基本相容。Acr-g-PU-PETA的热失重行为明显不同于共混材料，接枝样dTG峰明显宽化，表明接枝样中链段之间的相互作刚有多重性，丙烯酸树脂与聚氨酯之间有一定的相容性。接枝树脂自乳化体系光固化涂层的综合性能以聚氨酯丙烯酸酯成分为50％～30％者相对较好，且以Acr-g-TPU-PETA系列更理想。光固化膜的附着力为1～2级，冲击强度为50kg·cm，柔软性多为1mm。以HEMA代替PETA作为接枝树脂的活性基团，碳—碳双键密度太低，光固化膜交联点太少，硬度和附着力均较差。多羟基胺连硫杂蒽酮光引发剂能均匀分散于树脂自乳化体系和接枝树脂干膜中，不出现色斑，光固化膜肖氏硬度一般高于以Irgacure 2959为光引发剂的光固化膜，光泽度及耐丁酮溶剂性能均有所提高，不会诱导铁基生锈，钛白着色对光固化结果几乎没有影响。本论文所合成的多羟基胺连硫杂蒽酮光引发剂IV〓与IV〓为浅黄色，对光固化涂层的颜色干扰较小，为配制钛白着色光固化水性涂料提供了新的光引发剂。水溶性太强的WB 4759在干膜中易聚结析出，且对所附着的铁基有诱导锈蚀作用。

The paper also points out that, modern logistics can restructure and upgrade the regional industries by means of the effect of industrial relevancy when it serves for the social production and residents living, engenders new industries and enhances the internal proportions of the third industry. By integrating related functional operations, modern logistics is able to change the links between the knots and lines from random and loose connection to a kind of spatial structure of smoothness and steadiness, with which block and friction would be lessened and the function of regional economic constituents amplified while the regional income and the strength would be improved as a whole. Through intensifying the advantage of allocation of the centers of region, modern logistics induces regional elements of development to move towards advantageous locations while bringing key urban cities effects of scale economy and agglomeration economy, it then can be regarded as an operational platform for potentiating the growing polar of urban cities and boosting up the developing level of hinterlands, and an effective measure to balance urban and rural development and development among regions.

论文认为，现代物流在为社会生产和生活服务、形成新的产业形态、改善第三产业内部比例关系的同时，通过产业关联带动效应促进区域整体产业结构的优化升级；现代物流通过整合相关功能性活动，将经济网络中节点与线路之间随机连接、松散联系转变为顺畅交流、稳定合作的空间构造，从而减少区域经济运行中的阻滞性因素，放大区域经济发展要素的功能，提高区域经济系统整体效益和经济实力；现代物流通过强化经济中心的区位优势，促进区域经济发展要素向优区位运动，给经济中心城市带来规模效应、聚集效应和扩散效应，是强化中心城市增长极地位和提升经济腹地发展水平的操作平台，因而也是统筹城乡发展和区域发展的重要手段。

This paper mainly includes two parts The first part falls into three main sections. First, it analyses the formative causes of the Chinese traditional house interior display art through five aspects, including the structure and forms of Chinese traditional architecture, the productive mode of Chinese traditional society, Chinese traditional philosophy, the idea of ethics and grading and custom of Chinese traditional society. Second, it briefly introduces the history of Chinese traditional house interior display art's development. Finally, it focuses on the analysis of Chinese traditional house interior display art's design ideas and concludes that its design ideas embodies the five sides as follows: respect for nature and imitate artfully nature, practical-oriented and function incorporated into form, images of space of what the theory and practice forms, balanced natural pattern of the display, artifacts with the thoughts and respect for poetic imagery. Another part of the article further researches on the application of Chinese traditional house interior display ideas to the contemporary home display, and leads to the author's view, that is that each era should has its own character when it inherits the traditional culture. What's more, the contemporary Chinese-style home display design not only have to take over the spirit of the tradition but also have to give full consideration of the contemporary aesthetic concepts, principles and methods. In the 21st century, humanistic care is greatly proponed in the interior design. The interior display art should develop towards the ecological, personalized and comfortable direction and the cultural dimensions. It should absorb the traditional cultural elements to decorate contemporary living space. In order to upgrade the simple and primary "appearance likeness" to a higher level of "spirit likeness", it must effectively grasp the poetic imagery of space, which can reach the artistic effect of "both appearance and spirits".

本文的重点内容有两部分：一部分是从中国传统建筑的结构形式、中国传统社会的生产方式、中国传统的哲学观念、中国传统的伦理等级观念、中国传统社会的风俗习惯等五个方面简单分析了中国传统民居室内陈设艺术形成的原因；简要介绍了中国传统民居室内陈设艺术的发展过程；着重对中国传统民居室内陈设艺术设计理念的具体内涵进行了深入的分析，总结出其设计理念集中体现在五个方面，即崇尚自然与巧法造化、实用为本与文质彬彬、虚实相生的空间意象、均衡自然的陈设格局、器以载道与以境为尚；另一部分是对中国传统民居室内陈设设计理念在当代居室陈设设计中的应用作了较深层次的探讨，并提出了自己的见解，即每个时代对传统文化的继承都应该呈现出自己的特色，当代中式风格的居室陈设设计既要秉承传统民居室内陈设艺术的设计精髓，又要充分考虑当代审美观念、原则和方法的融入。21世纪，室内设计大力提倡人文关怀，具体到室内陈设艺术设计上，应该向生态化、个性化、舒适化和文化层面发展；吸收传统文化元素装饰当代居室空间，要有效地把握空间意境，使当代中式风格的居室陈设设计从简单、初级的&形似&上升到较高层次的&神似&，进而追求&形神兼备&的艺术效果。

The expression of HSP70 and HSP70mRNA and the activity of SOD and NOS in uterus were elevated with preventive acupuncture and preventive moxibustion and the influence on the activity of SOD and NOS was mainly refected in 12th and 16th month. It indicated that preventive acupuncture and preventive moxibustion on Guanyuan point could relieve the abnormal activity of center of stress, promote the expression of HSP70 and HSP70mRNA and the activity of SOD and NOS in uterus so as to maintain the normal structure and function and prolong the retrograde degeneration of uterus. It might be one of mechanisms of protecting uterus with preventive acupuncture and preventive moxibustion.

提示逆针灸关元穴对随后更年期大鼠子宫产生保护作用机制可能通过减缓应激中枢的异常活跃状态，提高不同月龄外周子宫组织细胞HSP70及mRNA的表达，以促进组织SOD、NOS活性的增强，对抗应激激素及卵巢功能衰退引起的子宫靶器官的结构及功能的改变，提高机体抗氧化能力，增强NO/NOS的局部调节作用，减轻随后更年期衰老过程中自由基对组织器官的损害及组织器官功能紊乱，对于维持子宫正常的结构和功能，延缓子宫退行性变具有重要作用。

Save, O Lord, and have mercy on the old and the young, the poor and destitute, the orphans and widows, the leper colonies, epileptics and spastics, and those in sickness and sorrow, misfortune and tribulation, captives and exiles, in mines and prisons and reformatories, and especially on those of Thy servants suffering persecution for Thy sake and for the Orthodox Faith from godless peoples, apostates and heretics.

主啊，求你拯救并怜悯老人和少年，穷人和贫乏者，孤儿和鳏寡，因患恶疾而遭隔绝的人，麻风病人，癫痫病人，瘫痪者，及所有身罹疾患痛苦的人，遭受天灾人祸者，身陷囹圄者，被充军流放者，服苦役者和受刑囚者；特别是为了正教信仰而遭受无神论者，叛道者和异端者迫害折磨的你的众仆役。

June 28. Having been somewhat refresh'd with the Sleep I had had, and the Fit being entirely off, I got up; and tho' the Fright and Terror of my Dream was very great, yet I consider'd, that the Fit of the Ague wou'd return again the next Day, and now was my Time to get something to refresh and support my self when I should be ill; and the first Thing I did, I fill'd a large square Case Bottle with Water, and set it upon my Table, in Reach of my Bed; and to take off the chill or aguish Disposition of the Water, I put about a Quarter of a Pint of Rum into it, and mix'd them together; then I got me a Piece of the Goat's Flesh, and broil'd it on the Coals, but could eat very little; I walk'd about, but was very weak, and withal very sad and heavy-hearted in the Sense of my miserable Condition; dreading the Return of my Distemper the next Day; at Night I made my Supper of three of the Turtle's Eggs, which I roasted in the Ashes, and eat, as we call it, in the Shell; and this was the first Bit of Meat I had ever ask'd God's Blessing to, even as I cou'd remember, in my whole Life.

这些我在故事一开始就提到了。父亲说，我如果执意采取这种愚蠢的行动，那么，上帝一定不会保佑我。当我将来呼援无门时，我会后悔自己没有听从他的忠告。这时，我大声说，现在，父亲的话果然应验了：上帝已经惩罚了我，谁也不能来救我，谁也不能来听我的呼救了。我拒绝了上天的好意，上天原本对我十分慈悲，把我安排在一个优裕的生活环境中，让我幸福舒适地过日子。可是，我自己却身在福中不知福，又不听父母的话来认识这种福份。我使父母为我的愚蠢行为而痛心，而现在，我自己也为我的愚蠢行为所带来的后果而痛心。本来，父母可以帮助我成家立业，过上舒适的生活；然而，我却拒绝了他们的帮助。现在，我不得不在艰难困苦中挣扎，困难之大，连大自然本身都难以忍受。而且，我孤独无援，没有人安慰我，也没有人照应我，也没有人忠告我。想到这里，我又大喊大叫：&上帝啊，救救我吧！我已走投无路了啊！&多少年来，我第一次发出了祈祷，如果这也可算是祈祷的话。现在，让我重新回到日记上来吧。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Those who loved reading were obliged to send for their books from England; the members of the junto had each a few

那些喜欢看书的人就得从英国购书，讲读俱乐部的的成员每人都有几本。

Three fourths of the earth's surface is covered with water.

地球表面的四分之三被水覆盖着。