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After the His·Tag fusion protein was cleaved by the enterokinase, intact biologically active PAT was released from the fusion protein and was purified to homogeneity with Ni2+ affinity chromatography .

用肠激酶切除了融合蛋白的融合部分之后再一次通过金属鏊和层析，经过透析后获得了 PAT 纯品。

The axe cleaved in front of heyward , cutting some of the flowing ringlets of alice

斧头从海沃德面前掠过，劈断了艾丽斯一些飘动着的头发。

My heart invariably cleaved to the master's, in preference to Catherine's side.

我的心情愿毫不更变地永远依附在主人身上，而不是在凯瑟琳那边。

No longer is the world cleaved in two, with developing countries roiled by infectious disease and industrial nations largely isolated from its scourges.

这世界不再一分为二，一半是受传染病所苦的开发中国家，另一半是不受其害的富裕国家。

Kunta's tongue seemed cleaved to the roof of his mouth.

昆塔的舌头好象粘在上腭了。

Methods From 1983 to 1992, 151 patients with diffuse large non-cleaved cell NHL were treated.

回顾性分析151例弥漫型大无裂细胞性非霍奇金淋巴瘤的临床资料。

The results of the experiments show that substituted aryl ethers that containing nitro group (-NO2) are easy to take place nucleophilic substitution in the alkaline medium and then aryl etherbond is cleaved.

实验结果表明，含-NO_2的取代芳基醚，在碱性溶液中，易发生亲核取代反应，从而使芳醚键断裂。

By controlling the potential of the electrolyte solutions,this technique can be employed in studies formation of DNA strand breaks.CT-DNA was cleaved by adding amounts of CuL or NiL complex in the absence of chemical reagent when appropriate electrode potential(sufficiently negative or positive to reduce or oxidate metal ions) was applied.

根据获得的实验结果，CuL或NiL存在下，电解溶液的电位合适时(足够负或足够正的电位，保证金属离子的还原或氧化)，不需要加入额外的氧化剂或还原剂，同样可以切割小牛胸腺DNA，CuL劈裂能力强于NiL配合物。

Simple and cleaved polyembryony were found frequently in the embryo development.

在胚发育过程中，既产生简单多胚，又产生裂生多胚。

The CPP32 was activated and the 32-KDa proenzyme was cleaved. The active subunit p17 was detected at 3, 6, 9h of incubation and reached the peak at 6h.

用ICE的特异性荧光底物Ac-YVAD-AMC分析ICE活性，证明ICE在整个过程中均未见升高，与正常细胞组相比无显著性差异。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。