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METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide or caspase-3 inhibitor (DEVD-CHO, 100 μmol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and IκΒ-α were detected by Western blotting methods.

用二甲基亚砜对照、100μmol/L caspase-3抑制剂预处理乳腺癌Bcap37细胞3h后，加入不同浓度的长春碱，以MTT法检测肿瘤细胞增殖能力，以细胞DNA片段分析及PI染色法检测肿瘤细胞凋亡，以蛋白免疫印迹法检测pro-caspase-3和IκΒ-α蛋白的变化。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明，在花粉母细胞减数分裂后的四分体解离时期，不育花药的绒粘层细胞较可育花药提前开始裂解；在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象，证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

The results showed that the rupture is transcrystalline cleavage cracking by hydrogen embrittlement basically,i.e.cathodic stress corrosion cracking.

指出管件破裂基本上属氢脆引起的穿晶型解理开裂，即阴极型应力腐蚀破坏。

After the addition of carbon, the microhardness in the grain boundary is about 150 MPa higher than that in the grain. The fracture type of pure Mo is intergranular fracture, the grain boundary strength of Mo-C alloy is strengthened due to the addition of carbon and the fracture type is changed to transgranular fracture, transcrystalline fracture and a large number of cleavage planes and steps are observed on the bending fracture surface. But if the carbon content is too high, the carbide precipitation will be severe at grain boundaries and is deleterioated to the ductility of the alloy.

添加碳后，合金的晶界显微硬度比合金的晶内显微硬度高150MPa左右；纯钼的断裂方式以沿晶断裂为主，添加碳后，由于合金的晶界强度得到提高，合金的断裂方式变成以穿晶断裂为主，断口上有大量的解理面，具有明显的河流状花样和解理台阶，且塑性越好的断口，撕裂岭也越多；当添加的碳含量偏高时，在晶界处产生了大颗粒的碳化物，这种大颗粒的碳化物会降低合金的塑性。

Two novel tridentate polypyridyl mixed-ligand cobalt [Co (H2Bzimpy)(superscript 3+)] and ruthenium complexes were synthesized and characterized via elemental analysis and (superscript 1)H NMR. The crystal structure of complex B was determined. The interactions between the complexes and calf thymus DNA were investigated with absorption and fluorescence spectroscopys. Photoactivated cleavage of pBR322DNA by the synthesized complexes were studied.

合成了两种新型三齿多吡啶钴[Co(H2Bzimpy)](上标 3+)和钌混配配合物，用元素分析和1HNMR等对其结构进行了表征，测定了配合物B的晶体结构用电子吸收光谱和荧光光谱等方法研究了配合物与小牛胸腺DNA的相互作用以及配合物对pBR322DNA的断裂作用。

In this sense, IDA plays dual effects, tridentate chelating to Cu(2) and bridgebetween two Cu(1) andCu(2).3 Metal complexes were selected as the appropriate for the study of cleavage plasmid pBR322DNA by gel electrophoresis technique. The results showed Ni and Mn complexes could cleave effect- ively DNA in the presence of H2O2 at physiological pH and temperature, whereas individual Zn complex could cleave effectively DNA.

通过电泳实验研究了一系列金属配合物与pBR322DNA的作用，发现在Tris-HCl缓冲溶液中，生理条件下，镍、锰配合物在共反应物H_2O_2存在下能够很好的断裂DNA，而Zn配合物单独作用就能够使DNA由ccc型转化为oc和linear型。

Coli, the C termini of outer-membrane proteins bind to the PDZ domains of the trimeric DegS protease and activate cleavage of RseA, a transmembrane transcriptional regulator.

ResA是一种跨膜的转录调控因子。

Then we compared cleavage rate and blastocyst rate among different groups. After that we evaluated the quality of blastocysts by using ICM/TE (Inner cell mass/Trophectoderm cells) ratio via staining with propidium iodide and Hoechest333258, and analyzed the level of genome methylation and histone acetylation by immunofluorescence.

比较各组受精卵的卵裂率和囊胚发育率；并应用碘化丙啶和Hoechest333258对囊胚进行染色，利用ICM/TE值评价各组胚胎体外发育的质量；同时将囊胚进行免疫荧光染色，观察其基因组甲基化和组蛋白乙酰化的水平。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明，T NF的两种形式在受体结合、生物学效应等方面各具特征［3，4］。M-TNF通过直接接触在组织局部中发挥效应的特性，有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

在克隆M-TNF cDNA的基础上，删除两型TNF转换酶酶解部位的编码序列，构建出mTNFm并测序验证；建立只表达膜型T N F的真核细胞株。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。