查询词典 cleavage

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

We propose that Aurora B is part of a sensor that responds to unsegregated chromatin at the cleavage site.

研究人员认为，Aurora B是位于分裂位点上，响应未分离染色体的感应器的一部分。

Objective To detect the human papillomavirus in biopsy specimens of cervical carcinoma and genital verruca by PCR-endonuclease cleavage.

目的 探讨人乳头瘤病毒(human papillomavirus，HPV)在宫颈癌的发生中的作用。

Objective This study was to detect the human papillomavirus and herpes simplex virus genotypes in biopsy specimens of cervical carcinoma and genital verruca by PCR-endonuclease cleavage.

近年来随着分子生物学技术在肿瘤病原病因学研究中应用，发现单纯疱疹病毒-2型和人乳头瘤病毒是引起妇女生殖器疣和诱发宫颈癌的重要病原之一［1～4］。

Methods Biopsy samples of were obtained from 353 patients with cervical carcinoma, HPV were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18 were found in 138 (39.1%) 353 cervical carcinoma, while HPV types 6, 11 were found in 81 (69.2%) of 117 acuminate verruca.

采用聚合酶链反应-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA，对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒(HPV6、11、16、18)的检测和分型。

This process of cell division or cleavage in the zygote is called segmentation.

这种细胞的分裂或受精卵的分裂称为卵裂。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同，滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%，显著高于昆明鼠(47.6%)；自发激活率分别为13.3%和46.0%，C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%，显著低于SrCl_2孤雌激活率(96.4%)；自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%)，但囊胚率(18.7%)较低，同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%，差异不显著。

Acylamino compounds from AD were synthesized by cleavage of A-ring through NaIO4/KMnO4 oxidation, ring closure by amination, oximation with hydroxylaminechloride in C-17 position, and reduction of oxime in C-17 position and substitution with substituted acylchloride and carboxylic acids.

本文以AD为起始原料，经过NaIO4/KMnO4氧化裂解、胺解环合、羟氨肟化反应，在甾体17位形成肟，并将肟还原成胺，与取代酰氯和有机羧酸反应，合成了15个酰胺化合物。

An interbedded sliding zone with fault-slip cleavage in an amygdaloidal basalt thin layer made up the embryo of the main sliding zone and the coupling chemical weathering with physical weathering based on lateral unloading made the interbedded sliding zone get a material prone to slide finally.

褶皱运动产生的构造裂隙与柱状节理的叠加使滑体玄武岩呈现碎裂—镶嵌结构，而沿结构面发生的化学风化形成的腐岩壳使岩体进一步转变为&石夹土&结构，加剧了坡体的时效变形。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。