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These results show that the dismutation activity is crucial for the supercoiled DNA-cleavage.

对酶化学修饰及热处理的结果表明两活性变化趋势一致。

An improved simple method for carbon-carbon double bond cleavage by silica-supported potassium permanganate was described and a possible mechanism was proposed.

在无有机溶剂做介质的条件下，用硅胶吸附的高锰酸钾氧化6种难溶于水的液态烯烃，得到了双键发生断裂的氧化产物。

I think you have to practice the fine art of cleavage glancing, without drooling .

我觉得瞥胸也要讲究艺术，看的时候不能流口水。

The main influential factors on surgical results were whether presence of encasement of the basilar artery and its branches and of the arachnoidal cleavage plane between the tumor and the brain stem.

肿瘤不能全切除和出现严重并发症的主要原因是肿瘤包裹了基底动脉及其分支以及肿瘤破坏了软脑膜使肿瘤与脑干间失去界面。

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD／TOPO中也得到高效融合表达，表达产物经酶切、纯化和复性，测得精氨酸酯酶活性。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

The expressed protein was enriched with TALON resin and the SLC was released with enterokinase cleavage,purified with weak cation exchange chromatography.

用羊抗人SLC多克隆抗体进行Western blot分析显示，在30 kDa 处出现单一阳性条带，而对照的pET32a/BL21trxB(DE3)诱导菌则无此反应。3。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。