查询词典 chymotrypsin

The results showed that the total numbers of integral membrane proteins identified based on PAOT,trypsin/chymotrypsin and methanol-modified digestions were 101,59 and 72,which included 67,40 and 31 proteins with GRAVY＞0,respectively.

结果表明，甲醇酶解法鉴定到72个整合膜蛋白，其中平均疏水值大于0的蛋白数为31；胰蛋白酶/胰凝乳蛋白酶酶解法共鉴定到59个整合膜蛋白，其中平均疏水值大于0的蛋白数为40个；PAOT法共鉴定到101个整合膜蛋白，其中平均疏水值大于0的蛋白数为67个。

Examples include trypsinogen and chymotrypsinogen, secrete d by the pancreas and converted by proteolysis in the small intestine into the active enzymes trypsin and chymotrypsin; and numerous coagulation factors.

这作用受不同的催化或受的活化型本身的催化。原细胞合成并贮存钝化型式的原。胰脏分泌的胰蛋白原及胰凝乳蛋白原在小肠内活化成胰蛋白及胰乳凝蛋白。

Examples include trypsinogen and chymotrypsinogen, secreted by the pancreas and converted by proteolysis in the small intestine into the active enzymes trypsin and chymotrypsin; and numerous coagulation factors.

原细胞合成并贮存钝化型式的原。胰脏分泌的胰蛋白原及胰凝乳蛋白原在小肠内活化成胰蛋白及胰乳凝蛋白。到中国译典经典版本中查找关于proteolysis的最新解释和例句。。。。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时，我们以人类CNE1细胞系为材料，比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法，发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍，并且这些整合膜蛋白的疏水值大部分都为正值。

In order to understand the mechanism of the insecticidal fraction F3-28 from the extract of Periploca sepium bark. The effect of F3-28 on the activities of protease, trypsin-like, chymotrypsin-like, lipase and amylase of the 5th larvae of Mythimna separata and Agrotis ypsilon were studied.

为了研究杠柳根皮中主要杀虫活性成分F3-28的作用机理，以载毒叶碟法饲喂小地老虎和东方粘虫5龄幼虫，分析试虫中肠摄食F3-28后蛋白酶、类胰蛋白酶、类胰凝乳蛋白酶、淀粉酶、脂肪酶的活性变化。

It revealed high inhibition specificity toward serine proteinases (bovine trypsin, bovine chymotrypsin, and porcine trypsin). It did not exert any inhibitory activity toward cystein proteases and aspartyl proteinases.

丝氨酸蛋白酶抑制剂对丝氨酸蛋白酶家族中的牛胰凝乳蛋白酶、牛胰蛋白酶、猪胰蛋白酶三种蛋白酶的活性均有强烈的抑制作用；而对半胱氨酸蛋白酶家族中的木瓜蛋白酶及天冬氨酸蛋白酶家族中的牛胃蛋白酶几乎没有作用。

Periploca sepium Bunge;protease;trypsin-like;chymotrypsin-like;lipase;amylase

杠柳；蛋白酶；类胰蛋白酶；类胰凝乳蛋白酶；脂肪酶；淀粉酶

Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路线使用α-胰凝乳蛋白酶和嗜热杆菌蛋白酶完成了N-保护的亮-脑啡肽的酶法全合成，并在第三戊醇中首次以嗜热杆菌蛋白酶为催化剂得到了Z-保护的亮-脑啡肽酰苯肼衍生物。

Among the midgut proteases in larvae, activities of chymotrypsin-like enzymes increased significantly with the increasing of Bt concentration, the activities of try...

在棉铃虫中肠内蛋白酶中，类胰凝乳蛋白酶的活力随Bt蛋白浓度增大而显著升高；类胰蛋白酶活性在Bt蛋白浓度为 2 0 μg g时达到最高，随后开始下降。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。