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Results indicated that suppression of malignancy was observed in the hybrid cells, they became differentiated morphologically and functionally by:(1) changes in histochemical staining, nucleus-cytoplasm in ratio, and increase in cell size and nucleal heterochromation, but decrese in cell surface microvilli and number of nucleoli;(2) decrease of growth rate, mitotic index and DNA synthesis rate;(3) loss of the ability to growth in soft agar medium, and non-tumorigenic under heterotransplantation to nude mice;(4) reduction of chromosome number mainly of rat derivative and decrease in chromosomal aberration rate.

实验结果表明：（1）杂交细胞出现瘤细胞与网织红细胞杂交同样的表型逆转及去恶性化特征，主要表现在细胞体积增大、核质比例减小；DNA合同速率降低、分裂指数下降，细胞生长显著减慢；在软琼脂中不形成集落，在裸鼠体内失去了长瘤能力。

Classically, the skin lesions occur perinatally in four successive stages: inflammatory vesicular (stage 1), verrucous (stage 2), hyperpigmented (stage 3), and scarring (stage 4), along the lines of Blaschko, reflecting the somatic mosaicism of X-chromosome inactivation in female patients.

典型的皮肤表现发生在周产期并可区分为四个阶段：发炎水泡，疣状增生，色素沉著和疤痕形成，而这些病灶都沿著Blaschko's line分布。它代表了女性患者身上一种嵌合型X染色体异常的现象。

Main parameters and methods of chromosome preparing with wall degradation hypotonic technique in cucumber were studied,and a suitable method was established.

以黄瓜卷须为材料，对黄瓜染色体制片中的一些主要参数和方法进行了研究，建立了黄瓜染色体制片的系统操作规程。

HPTTG could be up-regulated by γ-synuclein, impling that hPTTG might participate in this chromosome instability progression related with γ-synuclein.

综上所述，hPTTG的功能极其复杂，它参与细胞周期调控，沟通庞大的信号通路网络。

Results: Among 9 cases of long Y chromosome karyotyoe, 5 cases whose wives had infaust pregnancy history including abortion, embryo developing stop, dead embryo, etc.

结果：在检出的9例大Y染色体患者中，5例其妻有不良妊娠史（包括自然流产、胚胎停止发育、死胎等），3例性腺发育不全，1例46，XY男性性反转综合征。

Results:Among 9 cases of long Y chromosome karyotyoe,5 cases whose wives had infaust pregnancy historyincluding abortion,embryo developing stop,dead embryo,etc.

结果在检出的9例大Y染色体患者中，5例其妻有不良妊娠史(包括自然流产、胚胎停止发育、死胎等)，3例性腺发育不全，1例46，XY男性性反转综合征。

After 6 to 7 days of culture, Eleven ES cell lines were selected and explanted. At last we obtained 4,3,4 and 3 ES cell lines separately. One of the B6C3F_1×B6D2F_1 ES cell lines was selected to future study its competent for germline transmission.We founded the ES cell line has normal karyotype on the chromosome analyse.In teratoma experiment by injected ESCs into C57 mices inguen, we gained teratoma contained cells come from all three embryonic germ layers.

选择一株具有高度杂合背景的B6C3F_1×B6D2F_1 ES细胞系进行核型分析、畸胎瘤实验后与ICR品系2n胚胎构建嵌合体并得到具有生殖系传递能力的嵌合体小鼠，证明此细胞系具有生殖系嵌合能力。

Chromosome DNA was purified by isopycnic centrifugation in a CsCl-ethidium bromide.

氯化铯-溴化乙锭梯度离心法纯化DNA。

Tab 1 identification of mab value, isotype, chromosome and affinity constant

2.3 mab的特性鉴定 mab效价、亚类、染色体及亲和常数的鉴定，结果见表1。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！