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Nigra, B. oleracea and B. carinata belonged to class 2A according to the classification of karyotypes by Stebbins (1971). According to Levitzky's and Stebbin's view, the more asymmetric karyotype a species has, the more evolved it is. Therefore, B. campestris is the furthest evolved species among the elementary species, and B. juncea and B. napus the furthest among the amphidiploid species. Compared with the previous reports, there is no any cytoplasm in the chromosome sample prepared using this protocol which is very useful for the karyotype analysis, as well as the FISH research on Brassica species.

对上述研究结果分析表明：（1）芸薹属复合种是由基本种相互杂交而成；（2）B基因组与A或与C基因组亲缘关系较远，而A和C基因组亲缘关系较近；（3）芸薹属复合种在演化过程中，发生了染色体结构上的重排现象；（4）芸薹属复合种演化过程中，rRNA基因位点发生了减少；所获结果与国外同类报道相比表明：国内的白菜型油菜存在新的rRNA基因位点，即rRNA基因在芸薹属同一物种不同的品种间具有多态性；有关这类研究国内尚未见报道。

The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

Carthlicum, and was located on region of wheat chromosome 2AL near the gene Pm4b, it maybe either Pm4b, or a new gene linked to Pm4b. Temporarily, the gene was designated as PmPS5A. A dominant major gene conferring resistance to powdery mildew was identified in a BC〓F〓 population derived from a cross of amphidiploid Am9 and wheat cv. Laizhou953. The gene originated from T.

通过与含有已知抗病基因品种或品系的对该20个菌株反应模式比较和系谱分析，推测Am9／莱州953*〓 F〓含有Pm4b，波斯小麦PS5含有Pm4b与一个未知抗病基因组合；（DR147／Ae14）／／莱州953*〓 F〓和硬粒小麦DR147含有Pm4a和一个未知抗病基因组合；尾状山羊草Ae14和小伞山羊草Y39含有新的抗白粉病基因。

The F2 nullisomic amphidiploid with low chromosome number (2n=44-49) wasaccompanied with abnormal meiosis (7.45 Ⅰ+ 19.50 Ⅱ), but its fertility (19.2%) wasmuch higher than 〓's.

F2非整倍体的染色体数较低(2n=44-49)。

It"s estimated there"re some relations with hetero-sex-chromosome which male Amphioxus have.

估计可能与雄性文昌鱼具有异型性染色体有关。

In this study, the author used GISH technology to analyze the material that was resistant to take-all in test, and got three chromosome arm addition lines that carried the resistance genes derived from the amphiploid of Triticum-Haynaldia. The above arm addition lines and their resistant and sensitive parents were analyzed by RAPD method and two specific molecular markers that were tightly linked to resistant gene were obtained.

本研究先用基因组原位杂交对经鉴定抗全蚀病的材料进行分析，检测出三个抗性源于簇毛麦的端体异附加系材料，再利用RAPD方法，对这三个材料和抗感病亲本进行分析，得到了两个与全蚀病抗性基因紧密相关的特异性RAPD标记。

The chromosome constitution on H96276-2, which is a stable line derived from a sterile wheat-Agropyron intermedium partial amphiploid Zhong 3 cross to common wheat Hui 75, was analyzed by sequential GISH and FISH.

利用顺序基因组-重复序列原位杂交技术对1个来自中3不育系和普通小麦恢75杂种后代稳定株系H96276-2的染色体组成进行了分析。

C-banding and In-situ hybridization analyses of Ag.intermedium,a partiai wheat/Ag.intermedium amphiploid,and six derived chromosome addition lines 《TAG》 Friebe

八倍体小偃麦的形成，减数分裂稳定性和染色体组型的分析《作物学报》管启良 1980 // 03 P 129-137小偃麦新品种与中间类型的选育，程序和方法《作物学报》孙善澄

The recombinants with different multiple copies of amyL integrated in the chromosome were identified by real-time PCR and evaluated by shake-flask fermentation.

对转化子的amyL拷贝数及其BLA发酵水平分别用荧光实时定量PCR及摇瓶发酵试验进行评价与鉴定。

Synchronous and asynchronous chromosome separation was seen, and it was more common that the lagging chromosomes formed anaphase bridges.

有丝分裂过程中染色体的分离同步或不同步，不同步分离中的滞后染色体形成后期桥的现象更为普遍。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！