查询词典 chromogenic

Methods The t-PA mRNA in peripheral blood nucleared cells was detected by RT-PCR and the t-PA activity was determined by chromogenic substrate method.

用逆转录-PCR法检测外周血有核细胞中t-PAmRNA的表达，发色底物法测定血浆t-PA的活性。

［Purpose］ To explore the application for clinical assessment of HER2 gene expression status by chromogenic in situ hybridization and to compare the results with those obtained by immunohistochemistry.

［目的］探讨色素原位杂交在检测乳腺癌患者组织中HER2基因状态的临床应用，比较CISH与免疫组化检测组织HER2状态的差异性。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

During the research, Chaleted resin columm made in our laboratory was chosed for concentration, and ethylic violet is chose for chromogenic reagent.

论文通过实验对显色体系、浓缩体系和解吸体系进行了优化，分别确定了测定海水中痕量Cd和Cu的最佳分析条件。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

The chromogenic reaction of cetyltrimethyl ammonium bromidewith 2,4,6-tri nitrophenol was studied.

生成的缔合物在4 2 0 nm处有最大吸收，表观摩尔吸光系数为1.6×10 4 L·m ol- 1 ·cm- 1 ，苦味酸浓度在0～12 0μg·m L- 1 范围内符合朗伯-比尔定律，此法可应用于DDNP废水中硝基酚类的测定。

Methods Cyclohexane, ethyl acetate and glacial acetic acid (8∶8∶0.8) were used as the developing solvent. Samples were analyzed on a layer plate of silica gel G thin by using 20% phosphomolybdic acid as chromogenic agent.

以环己烷乙酸乙酯冰醋酸（体积比8∶8∶0.8）为展开剂，以羧甲基纤维素钠为黏合剂自制硅胶G薄层板，20％磷钼酸溶液为显色剂，进行薄层色谱分析。

To investigate the possibility of such a transition, we studied 17 cases of MGA or atypical MGA some of which had given rise to carcinoma in situ and/or invasive ductal carcinoma using the reticulin stain, immunohistochemistry (S-100, p63, Ki-67, and p53), and a molecular approach involving microdissection and high-resolution comparative genomic hybridization and MYC chromogenic in situ hybridization.

为了研究这种转变的可能性，我们分析了17例典型或非典型的MGA，其中一些已经发展成为原位癌和/或浸润性导管癌，所用的方法是网状染色、免疫组化（S-100， p63， Ki-67， and p53）和分子技术，分子技术包括显微切割和高分辨率比较基因组杂交及MYC显色的原位杂交。

Therefore, the nickel impurity in the chromium-plating bath was determined using spectrophotometry, which was based on the reaction between nickel ions and diacetyldioxime, generating a red complex, in the presence of alkali and ammonium per-sulfate. The measurement was carried out in 3cm color-matching cells at a wavelength of 510nm, using the sample without chromogenic agent as the reference.

在碱性和过硫酸铵存在的条件下，镍离子与丁二酮肟生成水红色配合物，利用这一特性，以丁二酮肟光度法测定镀铬溶液中镍杂质的含量，以不加显色剂的试样作参比液，用3cm比色皿，在波长510nm处测定。

Subsequently, the performance of the Pastorex Staph-Plus agglutination test with presumptive MRSA isolates taken directly from chromogenic agars was compared to that of the Pastorex Staph-Plus agglutination test with isolates from blood agar subcultures (conventional_Pastorex agglutination).

随后，我们采用Pastorex Staph-Plus乳胶试剂对显色培养基上被推断为MRSA的菌落进行直接乳胶凝集试验，同时采用接种在血琼脂上进行传代的纯培养菌落进行经典的乳胶凝集试验与之进行比较。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！