查询词典 chromatography

In the pretreatment group, Bak Foong Pill extracts were given for 14 days prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相-电化学方法检测纹状体巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相－电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Hours later,these mice were decapitated,and the striatums were isolated from thebrains. The levels of dopamine, serotonin ( 5-HT ), homovanillic acid, 5-hydroxyindoleacetic acid ( 5-HIAA ) andnorepinephrinein the striatum were measured by high-performance liquid chromatography with electrochemical detection.

给3组小鼠分别喂食Dieldrin 50 mg·kg-1体重、Dieldrin 40 mg·kg-1体重和等量的生理盐水，3 h后处死小鼠，取纹状体匀浆用高效液相色谱-电化学检测法测定纹状体中多巴胺、5-羟色胺(5-HT)、高香草酸、5-羟基吲哚乙酸(5-HIAA)和去甲肾上腺素的含量。

Immunohistochemistry technology was used to evaluated tyrosine hydroxylase of DA synthesis. DA, 5-hydroxytryptophan(5-HT) and their respective metabolic products 3,4 -dihydroxyphenylacetic, homovanillic are also precisely quantified in this research by using high-pressure liquid chromatography with electrochemical detector. The main metabolism monoamine oxidase of DA was processed too. RESULTS:WD can increase the synthesis and the metabolism process of DA .

本文观察了温胆汤及其配伍对大鼠刻板行为的影响，采用了免疫组化技术对多巴胺的合成酶—酪氨酸羟化酶进行了含量测定，应用高效液相-电化学法测定了DA、5-羟色胺（5-HT）及代谢产物3，4—二羟基苯乙酸、高香草酸的含量，并测定了DA主要降解酶—单胺氧化酶。

A method for the determination of hyaluronic acid in shark fin by high performance liquid chromatography is described.

建立了一种测定鲨鱼翅中透明质酸的酶解高效液相色谱方法。

A method for the determination of Acetic acid and Hydrobromic acid in air and environmental sample by ion-chromatography is described.

用同一吸收液采集、离子色谱法测定空气中的乙酸和溴化氢，采样体积为60L时，乙酸和溴化氢的检出限分别为0.03mg/m3和0.11mg/m3，相对标准偏差分别为1.9%和1.7%，实际样品的加标回收率分别为90.1%~102%和

Based on biological experiments, mango glycoside is used as raw material; under the condition with phenol and hydroiodic acid, carbon-carbon bonds are broken to produce the crude product of mango aglycone; the crude product is separated and purified through silica gel column chromatography so that the mango aglycone is separated; the yield rate reaches 12 percent, the purity is higher than 95 percent, and the reproduction performance is good; the IGTT of C57BL/6 normal mice is effectively improved with the norathyriol; and simultaneously the quantity of insulin secretion induced by glucose in vivo for the C57BL/6 normal mice can be improved with the norathyriol.

通过生物学实验证明，本发明所涉及的以芒果苷为原料，在苯酚和氢碘酸条件下，使得碳-碳键断裂，制备得到芒果苷元粗品，再通过硅胶柱层析分离纯化得到芒果苷元的方法使得其产率可达12％、纯度大于95％，且重现性好；norathyriol可使C57BL/6正常小鼠IGTT有明显改善；同时norathyriol可提高C57BL/6正常小鼠体内葡萄糖诱导的胰岛素分泌量。

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

Methods A rabbit was celiac-perfused by 5-Fu 100mg/Kg.Then assayed the durg concentrations of janitrix,abdominal aorta,peripheral venous,hydroperitoneum and tissues of liver and kidney by liquid chromatography in specific time;60 cases of hepatocellular carcinoma were radomized into experimental group and placebo group .

实验用家兔腹腔内注入5- Fu 100mg/kg，分别于设定时间抽取门静脉，腹主动脉，外周静脉血，腹腔液，肝、肾组织标本采用高效液相色谱法测定药物浓度；选择中晚期失去手术机会的临床确诊原发性肝癌病人60例，随机分组为实验组（行肝动脉化疗栓塞术中联合腹腔序贯化疗）和对照组（单纯行肝动脉化疗栓塞术）。

After extraction by ethanol and purification by cationic ion-exchange column, a method of using reversed-phase high-performance liquid chromatography and precolumn derivatization with o-phthalaldehyde and 9-fiuorenylmethylchloroformate is employed for determination of free amino acids in tobacco . This method assures that amino acids with amino and imino groups can be determined simultaneously. The results of duplication experiment and recovery experiment are acceptable.

本试验用乙醇提取烟叶中的游离氨基酸，并用阳离子交换树脂纯化，再用邻苯二甲醛、氯甲酸芴甲酯联合柱前衍生、反相高效液相色谱分析测定了烟叶中的游离氨基酸，该方法能同时测定烟叶中的带氨基和亚氨基基团的氨基酸，并且分析方法的重现性和回收率均令人满意。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。