查询词典 chromatography

Proteins associated with the membrane and the cytosolic portions of the proteome under different copper ion concentrations were differentially labeled with an acid cleavable isotope-coded affinity tag reagent, and the labeled peptides were separated by two-dimensional chromatography and characterized by electrospray ionization-tandem mass spectrometry.

我们分别将在不同铜离子浓度下的细胞质以及细胞内膜中所有蛋白质标识上酸裂性同位素亲和性标识(acid cleavable isotope-coded affinity tag， cICAT)试剂，用二次元管柱层析将所有标识并酵素水解过的片段分离，并且用电洒式串连质谱(electrospray ionization-tandem mass spectrometry， ESI-MS/MS)以及基因体定序资料库决定蛋白质序列。

A simple, rapid and accurate method for the determination of hydroxyproline in collagenous proteins by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was developed.

建立了一种简单、快速地测定胶原蛋白中羟脯氨酸的高效液相色谱-电喷雾质谱(HPLC-ESI/MS)方法。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA，测氨法测定 ADA活性，经SDS-PAGE测定其分子量。

And the content of capillary ion chromatography instrument includes micro-flow pump, injector, suppressors, conductometric detectors, UV-Vis detectors and fluorescence detectors with small volumes.

对毛细管离子色谱仪的总结包括微流量泵、小体积进样器、适合毛细管离子色谱系统的小体积抑制器、电导和光学检测器等。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析，以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量，并探讨了两种分析方法的精密度。911分子没有紫外吸收，为提高检测灵敏度，本文采用荧光标记法，通过对三种荧光试剂与几种寡糖和多糖衍生效果的综合比较，最终确定了盐酸胍为最佳衍生试剂，并以911为代表详细探讨了衍生化反应的最优条件。911糖链结构与褐藻胶主链结构类似，以甘露糖醛酸和古罗糖醛酸的嵌段形式存在，分子中M/G比值与911的物理化学性质和生物活性有直接关系。

And the content of capillary ion chromatography instrument includes micro-flow pump, injector, suppressors, conductometric detectors, UV-Vis detectors and fluorescence detectors with small volumes.

对毛细管离子色谱仪的总结包括微流量泵、体积进样器、合毛细管离子色谱系统的小体积抑制器、导和光学检测器等。

A simple and sensitive method was investigated for simultaneous determination six organic acids in wine (tatartic acid, citric acid, malic acid, formic acid, acetic acid and succinic acid) by single ion-exclusion chromatography with conductometric detection.

用单柱离子排斥色谱法分离结合电导检测同时测定了多种有机酸（酒石酸、柠檬酸、苹果酸、甲酸、乙酸和琥珀酸）。

A simple, selective and sensitive method was investigated for simultaneous determination of anions (SO2－4, Cl－, NO－3, F－) and cations (Na＋, NH＋4, K+, Mg2＋, Ca2＋) by single-column ion-exclusion-cation-exchange chromatography with conductometric detection. DL-malic acid-methanol-water was used as an eluent.

采用单柱离子色谱系统和电导检测的方法，首次以DL-苹果酸-甲醇水溶液为淋洗液，简便、有效地同时分离、检测了溶液中SO2-4、Cl-、NO-3、F-、Na+、NH+4、K+、Mg2+、Ca2+9种离子。

By a method using fraction precipitation of ammonium sulphate, ion exchange, modified hydrorylapatite and gel filtration chromatography, we separated and purified two kinds of phycoerythrin(R-PE and r-PE) from Corallina officinalis . The purity standard(ratio of ODs65/OD2go) of R-PE reaches to 11, which is the highest purity comparing with the reported purity of phycoerythrin.

经盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析等分离纯化方法，从珊瑚藻中分离纯化出两种藻红蛋白，其中R-PE纯度OD_(565／OD_(280)高达11左右，是目前国内外报道的藻红蛋白中纯度最高。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。