查询词典 chromatography

The AmkA, AmrA and AmrB had been purified with the His-Tag chromatography column.

其中，amrA，amkA和amrB基因的表达产物已经His-Tag亲和柱进行了纯化。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The experiment indicated,Using the anthrone color method observable knowledge sample sugar content is 8.8%,According to paper chromatography, Appraises its principal constituent using the anthracene for the glucose, the fructose, the mannose

实验表明，利用蒽酮比色法可测知样品的含糖量为8.8%，根据纸色谱法，鉴定出其主要成分为葡萄糖、果糖、甘露糖。

After the termination of the model,kill the mouse,detect NPY and then see the content of NPY in the Hypothalamus of the mouse significantly lower.3.8g/kg(it is valid dose proved by pharmacodynamic experiment,about 9 times of human dose) of Shanzha Maiya granula and 13.6g/kg(15 times of human dose) of Decoction of Xiangsha six Mild Drugs can play a certain role in treating the animal model of child apositia resulting from leptin,fail to increase the animal\'s food-intake,but can increase its weight to some extent,Decoction of Xiangsha six Mild Drugs can increase the content of NPY in the Hypothalamus of the mouse.4.set up quality standard of qualitatively and quantitatively detecting Shanzha Maiya granula:detecting the content of aurantiamarin as a main component of Chenpi in Shanzha Maiya granula through the method of high performance liquid phase,qualitatively identifying Shanzha,Maiya,Chenpi through the method of plate chromatography.

造模结束处死大鼠，放射免疫法检测NPY，结果造模大鼠下丘脑NPY含量显著降低(正常组下丘脑NPY平均含量为22.2ng/ml，模型组下丘脑NPY平均含量为14.8ng/ml，P＜0.05)。3。山楂麦芽颗粒8g/kg(药效学实验有效剂量，临床用量的9倍)和香砂六君子汤13.6g/kg(临床用量的15倍)对侧脑室注射瘦素致厌食症大鼠有一定治疗作用，虽没有提高动物进食量，但在一定程度提高动物体重，其中香砂六君子汤能提高大鼠下丘脑NPY含量(模型组下丘脑NPY平均含量为14.8ng/mL，香砂六君子汤组NPY平均含量为20.3ng/mL，P＜0.05)。4。

An efficient method for analyzing Arbutin and Hydroquinone in cosmetics by reversed-phase high performance liquid chromatography is developed.

探讨了高效液相色谱法同时测定美白化妆品中熊果苷和氢醌含量的方法，先对样品进行预处理，处理液为氯仿-饱和氯化钠溶液(体积比2∶1)，再选用Apollo C18柱，以甲醇-磷酸缓冲液-冰醋酸(体积比25∶475∶1)混合液为流动相，紫外检测，波长为254nm。

Objective To establish a thin layer chromatography for analyzing the components of bile arisaema.

目的 建立以猪胆汁制的胆南星的薄层色谱鉴别方法。

MethodsThe methanol extracts of the pericarp of Arnebia euchroma oil extract were separated with various chromatography technologies to produce some pure compounds and their structures were elucidated by spectroscopic and chromatographic methods.

方法利用色谱法对中药紫草油膏中的化学成分进行分离；利用核磁共振、质谱波谱方法对分离得到的单体化合物进行结构鉴定。

MethodsThe methanol extracts of the pericarp of Arnebia euchroma oil extract were separated with various chromatography technologies to produce some pure compounds and their structures were elucidated by spectroscopic and chromatographic methods.

方法利用色谱法对中药紫草油膏中的化学成分进行分离；利用核磁共振、谱波谱方法对分离得到的单体化合物进行结构鉴定。

This paper proposes a method for the determination of 4-hydroxy-3-nitro-benzenearsonic acid and arsanilic acid in the nitration reaction product of arsanilic acid by ion-pair chromatography.

本文提出一种用于测定对氨基苯胂酸硝化反应产物中4-羟基-3-硝基苯胂酸及对氨基苯胂酸含量的离子对色谱法，选择甲醇：水=25：75作为流动相，0。

A method for the simultaneous determination of arsanilic,nitarsone and roxarsone in animal feed was developed by anion solid-phase extraction and high performance liquid chromatography.

采用强阴离子固相萃取柱净化样品，建立了固相萃取/高效液相色谱法同时测定饲料中的阿散酸、硝苯砷酸和洛克沙砷的分析方法。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。