查询词典 chromatography

objective to determine the contents of residual acetone in acetoacetic ester,the raw material for the synthesis of edaravone, by gas chromatography.methods sample solution was injected on a gdx column (0.18 mm～0.25 mm) with nitrogen as the carrier gas.the residue was calculated by the external standard method.results the linearity was good over the range of 25 μg/ml～400 μg/ml,with the correlation coefficient of acetone being 0.996 and the average recovery 96.8%.conclusions the method was proved to be simple,rapid,practical and highly sensitive.it is an efficient method for the quality control of acetoacetic ester.

摘要］ 目的：用气相色谱法测定依达拉奉合成起始原料乙酰乙酸乙酯中丙酮的残留量。方法：采用固定相为二乙烯苯乙基乙烯苯型高分子多孔小球（直径为0.18 mm～0.25 mm），氮气为载气，用外标法测定乙酰乙酸乙酯中残留丙酮的含量。结果：该方法在丙酮浓度范围在25 μg/ml～400 μg/ml内呈良好的线性关系，响应曲线相关系数为0.996，平均回收率为96.8%。结论：本法简便，快速、实用，灵敏度高，可有效地用于乙酰乙酸乙酯的质量控制。

Objective To determine the contents of residual acetone in acetoacetic ester,the raw material for the synthesis of Edaravone, by gas chromatography.Methods Sample solution was injected on a GDX column (0.18 mm～0.25 mm) with nitrogen as the carrier gas.The residue was calculated by the external standard method.

摘要］ 目的：用气相色谱法测定依达拉奉合成起始原料乙酰乙酸乙酯中丙酮的残留量方法：采用固定相二乙烯苯乙基乙烯苯型高分子孔小球（直径为0.18 mm～0.25 mm），氮气为载气，用外标法测定乙酰乙酸乙酯中残留丙酮的含量。

Gas chromatography was employed to quantify the output of metabolization,and it was found that the yield of diacetyl for the mutant UV-3 was 18.7 fold to the wild-type 10293,up to 1.045g·L~(-1).Acetoin and ethanol yields were decreased by 48.4%and 71.4%,respectively.The yield of acetate was increased by 34.6%.

利用气相色谱测定代谢产物量，分析诱变前后菌株代谢途径中碳源的流向，结果表明突变株UV-3的丁二酮产量提高18.7倍，达到1.045g/L，乙偶姻产量降低48.4%，乙醇产量降低71.4%，乙酸产量提高34.6%，且遗传性质稳定。

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法，首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白，并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

Medicinal chemistry ; structural identification ; column chromatography ; Acrostichum aureurm ; chemical constituents

药物化学；结构鉴定；柱色谱法；卤蕨；化学成分

ABSTRACT To study the antibacterial constituents from the fermentation broth of endophytic fungus Penicillium sp. 0935030 from mangrove plant Acrostichum aureurm. The separation procedure was guided by antibacterial bioassay. Seven compounds were isolated and purified by column chromatography, and their structures were identified as cyclo (1), cyclo (2), 1(2Furanyl)1,2ethanediol (3),(3β,4β,22β)olean12ene3,22,23triol (4), mannitol (5), uridine (6), and liquiritigenin (7) by physicochemical and spectral data.

为研究红树林植物卤蕨内生真菌Penicillium sp.0935030发酵物中的抗菌活性成分，采用活性追踪的方法，用多种色谱技术对化合物进行分离纯化，通过理化性质和光谱数据鉴定出7个化合物，分别为：环(1)，环(2)，1呋喃基1，2乙二醇(3)，(3β，4β，22β)12烯3，22，23三羟基齐墩果烷(4)，甘露醇(5)，尿苷(6)和甘草素(7)。

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

Efficacious drugs were made of these species for treatment of cough, phlegm, breathe heavily, especially, to cure lung disease. We have investigated on the Chemical constituents of Adenophora stenanthina subspecies xifengsis collected from Gansu Qingyang, and twenty four compounds were isolated from ethanol extraction by chromatography technigues. Twenty compounds were identified by means of chemical and spectra methods（IR, FAB-MS, EI-MS,〓,〓, DEPT , among which three compounds were new as 3βvanilloyloxy-stigmast-5-ene-7β-one; 3β-Vanilloyloxy-stigmast-5-en-7 β-ol; 3β-vanilloyloxy-stigmast-5-en-7β, 15 〓-diol.

我们对采自甘肃庆阳的西峰沙参Adenophorastenanthina subsp.xifengensis）进行了化学成分的研究，利用现代层析手段从该植物的乙醇浸膏中分离出二十四个化合物，经化学方法和波谱技术（红外，质谱，核磁共振谱）鉴定了二十个化合物的结构，其中三个为新的化合物，它们分别是：豆甾-5-烯-7-酮-3β-香草酸酯；7β-羟基-豆甾-5-烯-3β-香草酸酯；7β，15ξ-二羟基-豆甾-5-烯-3β-香草酸酯。

After the establishment of irritable bowel syndrome model, the energy charge of adenosine triphosphate and the ratio of ATP to the total amount of adenylic acid pool in colonic epithelial cells at the ileocecum with better colonization were determined by high performance liquid chromatography.

在建立了IBS动物模型基础上，采用高效液相色谱法对CGC定植较多的回盲部肠黏膜上皮细胞的腺苷酸能荷及ATP与腺苷酸库比值进行测定，以反映细胞活性状态。

Methods The orthotopic liver transplantation in the rat was made by threecuffs. OLT rats were divided into two groups: OLT controland IP. The hepatic function and hepatic adenylic acids were determined by autochemical analysor and high performance liquid chromatography respectively.

方法制备三袖套大鼠同种异体原位肝移植模型，将其分为OLT对照组和IP组，用自动生化分析仪检测血中ALT、AST和LDH，用高压液相色谱仪测定移植肝组织中腺苷酸含量的变化。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。