查询词典 chromatography

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

High performance liquid chromatography, carbon-13 nuclea magnetic resonance, chemical ionization mass spectrometry and gas chromatography with flame ionization detection have been employed for the identification and quantitative deter mlnation of the epimers of amygdalin.

高效液相色谱法、carbon-13 nuclea 磁场共振电离质谱、化学品与气相层析法，以氢火焰离子化检测被用来识别和定量的威慑 mlnation差向异构体的苦的。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The purity of refined Auricularia auricula water-soluble polysaccharides was checked by gel filtration chromatography of Sephadex G-200 and gel permeation chromatography.

凝胶色谱Sephadex G-100和凝胶渗透色谱法检验得到的峰形都为单一对称峰，表明得到纯度较高的黑木耳多糖AAP3-1。

The liquid chromatography can be subdivided into high performance chromatography and several chromatographic methods running under normal pressure.

液相有可以细分为高效液相方法和几种常压的液相方法。

The gas chromatography and gel permeation chromatography methods for determining the residues of 2,4-dichlorophenoxyacetic in bean sprout was developed.

建立了豆芽中2，4-二氯苯氧乙酸残留量的凝胶渗透色谱净化——毛细管柱气相色谱测定的方法。

Methods Capillary gas chromatography and FFAP capillary column were applied; active carbon tube was used to collect the organic solvent samples in the air of workplaces, the samples were dissolved with carbon bisulfide and determined with capillary gas chromatography.

采用毛细管气相色谱法，选用FFAP毛细管分离柱，用活性炭采集工作场所空气中有机溶剂，经二硫化碳解析后进样测定。

High temperature liquid chromatography is a new field in the development of chromatography.

高温液相色谱是色谱发展的一个新领域。

In this paper, with the framework of non-equilibrium thermodynamic separation theory, a mathematical model of nonlinear chromatography is established according to the actual chromatography processes based on the three basic theorems, Lagrangian-Eulerian description, continuity axiom and local equilibrium assumption.

本文在梁恒等建立的非平衡热力学分离理论的框架下，以Lagrangian-Eulerian描述、连续性公理和局域平衡假设三大基本法则为基础，建立了一种制备色谱理论模型。

In the study, protein components in cottonseed meal and enzymatic hydrolysates of CSM were analyzed by general methods, gel filtration chromatography and high performance liquid chromatography.

通过常规测定、凝胶层析、高效液相色谱等3种方法，测定和分析了棉粕蛋白及其枯草蛋白酶酶解产物的组份，并进行比较研究。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。