查询词典 chromatography

An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

Thermophile was purifided to homogeneity, by using ammonium sulfate fraction, DEAE-Sepharose chromatography, Phenyl-Sepharose chromatography and Sephacryl S-100 chromatography.

粗酶液经硫酸铵分级沉淀、DEAE-Sepharose阴离子层析、Phenyl-Sepharose疏水层析、SephacrylS-100分子筛层析等步骤获得凝胶电泳均一的内切β-葡聚糖酶。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

To our knowledge, reverse phase of high performance liquid chromatography was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.

本试验首次确立了γ-生育酚代谢物的RP-HPLC分析方法，结果理想且重现性好；用GC／MS对γ-生育酚的未知代谢物结构进行分析，判断其中一种主要的代谢物为γ-生育酚侧链初级羟基化代谢物；γ-生育酚在雄性SD-大鼠肝S9的代谢动力学表明，本实验条件下代谢反应的最佳时间为45min，S9浓度为5mg／ml，NADPH浓度为1mM，γ-生育酚浓度为8μg／ml；发现γ-生育酚在雄性SD-大鼠肝S9中代谢最高，雄性巴西龟次之，雄性军曹鱼最低。

Methods Purification results were compared among the differential combination chromatography of anion and cation exchange, anion and cation exchange chromatography of both isocratic elution, and affinity chromatography.

阴、阳离子交换示差组合色谱法，以等度洗脱方法进行的阴、阳离子交换组合色谱，以及亲和色谱的纯化结果相对比。

As to the high polarity, high boiling point resultant—trimesic acid, the analysis methods of infrared spetrum, gas chromatography-mass spectroscopy, vapour-phase chromatography, acid number titration and so on are seperately used to characterize its property. Infrared spetrum and gas chromatography-mass spectroscopy are used to have a qualitative analysis on the resultant and are compared with the stand diagram. Sulfuric acid-methanol esterification and rapid esterification are used to prepare the trimesic acid trimethyl-ester and have a FID quantitative analysis. So that we make sure the purity of trimesic acid in the resultant and also we have an acid alkali titration on the resultant to get its acid number, in order to verify the purity of trimesic acid in the resultant.

对于高极性、高沸点的固体产物均苯三甲酸，分别采用红外光谱、气质联用、气相色谱、酸值滴定等分析手段进行了表征：利用红外光谱、气质联用的方法进行产物的定性分析，并与在同一仪器、同一条件下做出的标准谱图进行比对；采用硫酸-甲醇酯化法和快速酯化法制备出均苯三甲酸三甲酯，对其进行气相色谱FID定量分析，进而确定固体产物中均苯三甲酸的纯度；用酸碱滴定法确定固体产物的酸值，以对固体产物中均苯三甲酸的纯度进行验证。

Moreover, The taxol of zymolytic extraction is analyzed by Thin layer chromatography, High performance liquid chromatography, Micellar electrokinetic chromatography and MALDI-TOF.

并对发酵提取物内的紫杉醇用TLC、HPLC、毛细管电泳和MALDI—TOF进行了定性定量分析。

Advances in the separation of metal chelates by liquid chromatography, including thin-layer chromatography and column chromatography were reviewed and their applications in analysis of metal ions were given as will.

综述了用薄层液相色谱法和柱液相色谱法分离金属螯合物的进展及其在金属离子分析方面的应用。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径：发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

For the determination of five carbamate pesticide residues by high performance liquid chromatography with gel permeation chromatography purification, sample was extracted with acetonitrile and purified with gel permeation chromatography. Methanol-water was used as the mobile phase by gradient elution, flow rate of 1.0mL/min, using a fluorescence detector and quantitating with external standard method.

凝胶渗透色谱-液相色谱法测定5种氨基甲酸酯类农药残留中，实验采用乙腈提取，凝胶渗透色谱柱对样品进行净化，以甲醇-水为流动相，进行梯度洗脱，流速1.0mL／min，荧光检测器检测，外标定量测定。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。