查询词典 chromatography

The chromatography is carried out either at a constant temperature or according to a given temperature programme.

气相色谱法可以在常温下进行，也可以根据给定的温控程序进行。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

A liquid chromatography-electrospray tandem mass spectrometry was developed for the determination of trace amount of abscisic acid in plant samples.

脱落酸是植物体内产生的一种植物激素，它参与调控植物的生理过程[1]，其主要作用是促进芽休眠、叶子脱落，抑制生长。

The process avoids complex operation such as column chromatography, high pressure and absolute conditions.

本方法避免了柱色谱分离，高压操作，无水操作等复杂的工艺过程。

As endogenous jasmonates exist in enantiomers in nature and at level of nanogram/per gram fresh weight, it is very challenging work to develop rapid and accurate method for qualitative and quantitative analyses of JAs. This review paper summarizes the recent advances in the analysis of jasmonates including chromatography, capillary electrophoresis, chromatography-mass spectrometry and immunoassay.

内源茉莉酸类激素通常以多种手性异构体存在，且含量超微，因此，对内源性茉莉酸类激素进行准确的定性、定量分析具有很大的难度，研究高效分离富集、高灵敏度检测以及分离与检测联用等分析方法，对加速植物激素分子作用机理研究具有重大意义。

The fatty acids composition in the Pine seed oil were determined by means of gas chromatography and gas chromatography -mass spectrometry. The unsaturated fatty acids amounted to 90% of total fatty acids, in which 62% is essential to human health.

用气相色谱及气相色谱-质谱连用技术分析了红松仁油的脂肪酸组成，结果表明：不饱和脂肪酸的相对含量为90％，其中人体必需脂肪酸占62％左右。

In order to adapt to market development, and continuously enhance our wine advantages, the company at the original investment on the basis of more than 5,000 million, in the scenic Jiao Town, Gaoyao City covers an area of 160 acres to build the new plant, the introduction of modern wine equipment, with modern cooking wine shop and packing plant, and in December 2005 through drug GMP certification, with cleanliness standards of GMP 100,000 of wine production workshop, the use of liquid chromatography and gas chromatography for each Product the quality aspect of strict monitoring, product quality supervision department Detected by the passing rate was 100%.

为了适应市场的发展，不断增强我们的酿酒优势，公司在原来的基础上投资5000余万元，于风景秀丽的高要市蛟塘镇兴建占地面积160多亩的新厂房，引进现代化的酿酒设备，拥有现代化的煮饭酿酒车间及包装车间，并于2005年12月通过药品GMP认证，拥有洁净标准达十万级的药酒GMP生产车间，使用液相色谱和气相色谱对产品每个环节的质量进行严格监测，产品经质监部门检测合格率达100%。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

Results Method I of gas chromatography can't be used for determination of monosaccharide in spirulina glucoprotein, while method 2 was suitable, and 7 monosaccharides were found in ion chromatography.

结果 气相色谱法1不适于藻糖蛋白单糖分析，气相色谱法2较适合藻糖蛋白单糖分析；离子色谱法测得藻糖蛋白含7种单糖。

The purification method of the deoxyribonuclease comprises the following steps: drying fresh fruiting body of Ganoderma lucidum, pulverizing, extracting to obtain crude deoxyribonuclease, salting out with ammonium sulfate, carrying out affinity chromatography, gradient-eluting, carrying chromatography in SP Sephorose Fast Flow column, and gradient-eluting with 0 to 0.3 M NaCl to obtain seoxyribonuclease of Ganoderma lucidum.

该酶的提取纯化方法，以新鲜灵芝子实体为原料，经干燥处理后粉碎、粗酶抽提、硫酸铵盐析、亲和层析，梯度洗脱，再用SP Sephorose Fast Flow柱层析，以0～0.3M NaCl梯度洗脱，即可获得灵芝脱氧核糖核酸酶。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。