查询词典 chromatin

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P＜0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P＜0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果：与单纯抗甲状腺药物治疗比较，患者经抗甲状腺药物与中药联合应用治疗2-10周后，甲状腺组织明显缩小(P＜0.01)；光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态；加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P＜0.01)；末端标记呈阳性。

RESULTS: TEM displayed normal morphology of EC, its conjunction, SMC and intimal elastic lamina in control group, while in SAH group, marked pathological changes were observed, including desquamation and dystrophy of ECs, disruption of tight junction, vacuolated cytoplasm, condensation of chromatin in ECs, corrugation of IEL, and distortion of SMC.

结果： 电镜下，对照组血管壁超微结构无变化；SAH组电镜下血管内皮层结构以及内皮层细胞结构发生严重变性改变；用药治疗和预防组电镜下各组之间血管超微结构的改变无明显差异，组织变性程度明显轻于SAH组。

Cerebral angiography demonstrated basilar arteries spasm on SAH Day 5.(3)TEM demonstrated desquamation of endothelial cells, chromatin condensation, vacuolated cytoplasm and other morphological changes similar to atypical apoptosis.

脑血管造影见CCA结扎后兔基底动脉迂曲、扩张、延长，颅内外侧支循环增多；（2旧AH后所有动物的饮食量下降，神经功能出现障碍，oBF亦下降，以第4～5天为著。

RESULTS: TEM displayed normal morphology of EC, its conjunction, SMC and intimal elastic lamina in control group, while in SAH group, marked pathological changes were observed, including desquamation and dystrophy of ECs, disruption of tight junction, vacuolated cytoplasm, condensation of chromatin in ECs, corrugation of IEL, and distortion of SMC. In other groups, TEM showed slight ultrastructural changes in EC, SMC and IEL.

结果： 电镜下，对照组血管壁超微结构无变化；SAH组电镜下血管内皮层结构以及内皮层细胞结构发生严重变性改变；用药治疗和预防组电镜下各组之间血管超微结构的改变无明显差异，组织变性程度明显轻于SAH组。

Fig 5 In experimental group, mitochondrion in SO cell congregated at one end and nuclear with vacuolization and concentration of chromatin congregated at brim of nuclear

图4 实验组SO细胞肿胀，细肌丝疏松，排列紊乱致细肌丝结构不清。密体分布不均，形态大小及其长轴与细肌丝排列方向成角

In eukaryotic cells, the fundamental unit of chromatin is nucleosome, which is composed of a histone octamer with two copies of H2A, H2B, H3 and H4 and 146 base pairs of DNA.

真核细胞中，核小体是染色质的基本单位，它由两个拷贝H2A、H2B、H3和H4所组成的核心组蛋白八聚体，及缠绕于其上的146bp DNA构成。

Including the common component CBP/p300, one of whose functions is to modify the structure of chromatin by acetylating

它的名字最初来自于果蝇上的同源位点（这个基因决定果蝇身体结构的特征）。

"Acetylating H3K4 and demethylating H3K9 can actiate a gene; deacetylating H3K4 and methylating H3K9 can silence it," Karpen explains."Multiply modified at arious locations, these histones are a major factor in changing the functions of chromatin, independent of DNA sequence."

&H3K4的乙酰化和H3K9的去甲基化使基因活化；而H3K4去乙酰化和H3K9的甲基化则使基因沉默，& Karpen讲解道，&组蛋白的多部位多重调节是改变染色质功能的主要因素，并与DNA的序列无关&

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。