查询词典 chromatin

SAGA is a multifunctional ATP=independent chromatin remodeling complex which is composed of at least 20 proteins. It can acetylate N=terminal lysines of nucleosomal histone H3 and H2B to loose chromatin structure and promote transcription initiation.

SAGA是一个至少由20个蛋白组成的不依赖ATP的多功能染色质重塑复合物，它通过对组蛋白H3和H2B氨基末端赖氨酸乙酰化修饰来松动染色质结构，从而促进基因转录的起始。

In chromatin immunoprecipitation assay, we found that CBP/p300 could acetylate histones on IL-5 promoter, resulting in increased accessibility of the open chromatin to the transcriptional machinery and therefore promoted gene transcription.

这样产生一个相对开放的染色质构型，从而促进转录机器与DNA的结合，进而促进转录。

Results Lactacystin dealed with C6 glioma cells in 24 h, kytoplasm was concentrated and anachomasised, the chromatin of nucleus was concentrated pyknoticly to see under photo-microscope. The microvilli on surface dispeared、nucleus crenated、chromatin aggregated pyknoticly under transmission electron microscope respectively.

结果 Lactacystin处理体外C6胶质瘤细胞24 h后，光镜下见胞浆浓缩深染，胞核染色质致密浓缩，透射电镜下见细胞表面微绒毛消失，核皱缩，染色质致密浓缩、边集。

Results HE staining showed that sulindac could inhibit the growth of tumor cells, with the morphological presentations as apoptosis and necrosis including pyknosis, karyorrhexis and chromatin crescent formation, etc. By electron microscopy, cells also had apoptotic characteristics including chromatin crescent formation and apoptotic body. Immunohistochemistry and Western-blot assay results showed that 48 hours after sulindac administration, the expression of NF-κB, bcl-2 and bax gradually decreased, while the level of bax protein gradually increased, in a dose dependence manner.

结果 苏木精－伊红染色检测结果显示舒林酸抑制肿瘤细胞生长，肿瘤细胞出现核固缩、核碎裂、染色质边集等凋亡及坏死的形态学表现；电镜下可看到染色质边集、凋亡小体；免疫组化、Western-blot结果显示舒林酸作用48 h后人胃癌SGC-7901细胞NF-κB、bcl-2表达逐渐下降，bax表达逐渐上升，具有剂量依赖性。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化：染色质固缩边聚，形成致密、均质的染色质块，胞质浓缩、电子密度升高并空泡化；凋亡巨噬细胞溶酶体、线粒体增多；细胞突起缩短减少，但巨噬细胞特有的板状伪足不消失。

Using electronic microscope we not only observed the change of apoptotic cell chromatin such as chromatin condensing and margining but also watched the change of mitochondrias.

本实验通过电镜观察到凋亡细胞染色质聚集、趋边等表现，同时也观察到线粒体的改变。

Electron-microscopic observation: In stroke-prone spontaneous hypertension group, electron density was found increased in necrotic neurons; moreover, some nuclear membrane lost double-layer structure with ridges broken, even reduced or disappeared, displaying vacuolated changes. In losartan 30 mg/ group and losartan 10 mg/ group, most of neurons displayed basically normal morphology, with neuron chromatin evenly distributed and nuclear envelops regular, but there were still some neurons that had dense chromatin, with ridges broken and reduced.

3组大鼠脑组织电镜观察：易感型自发性高血压组大鼠坏死神经元电子密度增高，有的核膜失去双层结构，嵴断裂、减少甚至消失，呈空泡样改变；氯沙坦30mg/组和氯沙坦10 mg/组大多数神经元染色质分布均匀，核膜规整，细胞形态基本正常；少数神经元核染色质较致密，部分嵴断裂、减少。

Histone acetyltransferase CBP/p300 remodels chromatin by acetylating histones resulting in open structure of chromatin and active transcription.

转录辅因子CBP／p300本身具有组蛋白乙酰转移酶活性，参与许多基因的转录调控过程。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

To understand the listed contents:the studies about component of nuclear pore complex; the main functions of gp210;the different configuration models of sequence specific DNA-binding proteins; karyotype and chromosome bandings; configuration of chromatin and dene transcription; nuclear bodies is many subnuclear domains which have different conformation between chromatin.

了解核孔复合体成分的研究；gp210主要的功能；序列特异性DNA结合蛋白的不同结构模式；核型与染色体显带；染色质结构与基因转录；染色质之间的空间发现的许多形态上不同的亚核结构域——核体。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。