查询词典 chromatin

Morphological observation of U937 cells, treated with 0.2mg/ml, 0.4mg/ml,0.6mg/ml matrine by light microscope, electron microscope and image analysis, showed reduction of karyoplasmic ratio, chromatin margination,and presence of apoptotic body,compared with the control group.

0.2mg/ml、0.4mg/ml、0.6mg/ml Mat均可使U937细胞核浆比例下降，染色质边集，出现凋亡小体，随Mat浓度增加细胞形态学改变越明显。

Ultraviolet radiation can induce HaCaT cells apoptosis.One of morphological feature of cells apoptosis is karyopyknosis and clumped chromatin and formming circularity or crescent.

紫外线辐射HaCaT细胞可诱导凋亡，细胞发生凋亡的形态学特点之一是细胞核固缩，染色质浓缩，凝集，形成环状或新月体结构。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤（10，15 gkg-1d-1）治疗后，异位内膜有较多腺上皮细胞出现凋亡的特征，表现为细胞体积变小，核固缩，胞浆和核染色质凝集，密度增高，细胞间凋亡小体，同时间质细胞中可见一些坏死细胞；异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

Results When the cells were treated with Sodium Selenite at a concentration of 2.0mmol//L lasting 72h, karyopyknosis and chromatin condensation were observed under microscope; When the cells were treated with Sodium Selenite at a concentration of 4.0mmol/L lasting 72h, the cells in G0/G1 and S phase was significantly decreased, a typical apoptosis peak before G1 phase was shown.

结果 2.0mmol/L亚硒酸钠作用72h， HE切片可见核固缩、染色质边集等改变；4mmol/L亚硒酸钠作用72h， G0/G1期和S期细胞明显减少，在细胞周期G1前期可见典型的细胞凋亡峰。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ（ P .05）. There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%（48h after transfection） to 5.63%（72h after transfection）.

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制（ P 。05）；细胞形态发生改变，细胞体积缩小，核固缩，染色质浓缩聚集于核膜；流式细胞术检测显示G 1 峰前出现亚二倍体峰，转染48、72h后凋亡率分别为5.47%和5.63%（ P nbsp； 0.05）。

Typical characteristics of apoptotic cells as membrane blebbing, chromatin pyknosis, karyorrhexis, and apoptotic body were seen under electromicroscope. Apoptosisspecific DNA ladder bands from SGC-7901 cells were visualized with electrophoresis. Hypodiploid apoptotic peak was observed before G1 phase by flow cytometry analysis.

凋亡细胞形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成；DNA电泳呈现凋亡特征性阶梯状条带；流式细胞仪分析显示G1期前出现亚二倍体凋亡峰。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

A 30 min exposure to peroxynitrite (10〓μmol/L) followed by incubation in fresh medium induced apoptosis in cultured cerebellar granule cells, which was confirmed by DNA laddering, chromatin condensation, nuclei fragmentation, and the formation of apoptotic bodies.

原代小脑颗粒细胞经10μmol/L过氧化亚硝基处理30min，并继续培养一定时间后逐渐发生细胞凋亡，主要表现为DNA链断裂、染色质凝缩、核破裂、形成凋亡小体。

Only Lamin A was observed to be able to be co-immunoprecipitated with 8B7 spectrin.Lamin A is a component in lamin and functions as a common anchoring site for many proteins.It plays important roles in maintaining normal morphology of nuclear envelope,presenting anchoring site for chromatin and reconstructing of nucleus.8B7 spectrin may function through interaction with Lamin A.

Lamin A是核纤层的组成成分，由LMNA基因编码，是核内许多蛋白分子的共同锚定物，在维持核膜的正常形态，提供染色质的锚定位点以及在核的重组过程中可能发挥重要作用。8B7血影蛋白可能经由Lamin A发挥功能。

Fig.10 Showing K562 cell line treated with CA for 24h:an apoptotic cell with condensed chromatin marginated in crescents around the nuclear envelope.

图10 示2μm/L CA作用24h，加药组中1个凋亡的K562细胞，可见其染色质靠核膜边集，呈新月形

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。