查询词典 chromatin

The concept of chromatin and chemic compose; chromatin proteins; the essential about the structure of nucleosome; euchromatin and heterochromatin.

二 染色质的概念及化学组成；染色质蛋白质；核小体结构要点；染色质包装的结构模型；常染色质和异染色质。

The neuron apoptotic cells were observed by their fragmented nuclei condensed chromatin, fragmented or condensed cytoplasm and floccule of mitochondrion in the control group after ischemia 90 min/ reperfusion 12 h; whereas the distribution of chromatin was well-proportioned, and normal mitochondrion and other organelle were observed in the Ginkgo Biloba Extract-treated group.

对照组缺血90 min再灌注12 h鼠脑组织电镜检查可见神经元细胞核染色质边聚，呈团块状，线粒体可见絮状结构改变；相同时限药物治疗组脑组织神经元细胞核染色质分布比较均匀，胞质内线粒体及其它细胞器基本正常。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明：纤毛虫形成包囊过程中，皮层细胞质中产生具有自噬泡消化特征的细胞器，将部分纤毛结构等逐步消化；休眠包囊中，表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体，大核染色质和核仁颗粒变小，核孔增大，并发生染色质附着于核孔内膜的现象；细胞脱包囊期间，皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化。

Several masses of condensed chromatin without nuclear membrane were observed in some treated K562 cells,while chromatin of some other cells tended to marginate in crescents around the nuclear envelope with compacting cytoplasm,normal or slightly expanded endoplasmic reticulum,which presented typical morphology of apoptosis.

电镜下，加药处理组中部分细胞染色质凝集成块，没有核膜包被，部分细胞染色质靠核膜边集呈新月形，胞浆浓缩，内质网结构正常或有扩张，呈现典型的凋亡细胞形态。

We transformed antiapoptotic genes Bcl-2, CED-9 and PpBI-1 into the yeast to evaluate their function of resistance to cold stress. The results are as follows:(1) Cold induced apoptosis in yeast cells, moreover, yeast cells display representive features such as chromatin condensation, DNA fragmentation and chromatin margination.

而且酵母没有如Bcl-2家族的主要凋亡调控基因的同源基因，因此酵母是研究外源抗凋亡基因Bcl-2，CED-9和PpBI-1在抗冷方面的作用及其调控机制的理想体系。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明：百合花粉母细胞中有类似核纤层的结构存在；在染色质穿壁运动过程中，首先是细胞核向细胞壁靠拢，并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域；其后是染色质开始穿壁，在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域，到了染色质穿壁后期，大部分核物质都已穿至相邻细胞，并彼此融合，而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。