查询词典 cholera hog

This paper had obtained the data related to porcine reproductive and respiratory syndrome,hog cholera and porcine circo virus by questionary , locale inquire and sample detecting for all kind of scale hogpen in Henan province.

通过对河南省不同规模猪场问卷调查、现场问询调查和采样检测，获取有关猪瘟、猪繁殖障碍与呼吸综合征、圆环病毒病等疫病流行病学的相关情况。

The results showed that porcine reproductive and respiratory syndrome virus positive hogpen was 34.4%, hog cholera virus positive hogpen was 41.7%, PCV-Ⅱ positive hogpen was 36.2%; the percentages of coinfections of CSFV with PRRSV, CSFV with PCV-Ⅱ was 24.54% and 37.79%, PRRSV with PCV-Ⅱ was 36.87%.

结果表明，PRRSV阳性猪场为34.4%，CSFV阳性猪场为41.7%，PCV-Ⅱ阳性猪场为36.2%；CSFV与PRRSV、PCV-Ⅱ混合感染分别占CSFV感染的24.54%和37.79%；PRRSV与PCV-Ⅱ混合感染占PRRSV感染的36.87%。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.

对 2 0日龄 SPF猪和 2 0日龄猪繁殖与呼吸综合征血清阳性猪，人工感染猪繁殖与呼吸综合征病毒北京分离株( BJ-4 )后 4 8h接种猪瘟疫苗，利用 ELISA方法检测仔猪针对 PRRSV和猪瘟疫苗的体液免疫，利用 MTS法检测仔猪外周血单核细胞对有丝分裂原 Con A的刺激反应。

From January 21306 to December 2077,1 169 pigs were investigated from those taken to Zhengzhou College of Animal Husbandry Engineering Animal Hospital for diagnose to judge the immune state of porcine contagious pleuropneumonia, and to detect the infection of hog cholera virus, porcine circovirus, porcine reproductive and respiratory syndrome virus, pseudorabies virus and toxoplasm.

方法］2006年1月至2007年12月份历时2年，郑州牧业工程高等专科学校附属兽医院对来诊的未进行传染性胸膜肺炎疫苗接种的1169头猪随机采血，进行猪传染性胸膜肺炎抗体水平检测。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失