查询词典 cho

ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞（cDNACHO，采用MTT比色法检测1-甲基-4-苯基吡啶离子（MPP^+对CHO细胞野生株（wtCHO和cDNACHO细胞的毒性作用，并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

There is a relative shift in substrate utilization from CHO to fat and a better abdominal discomfortable when consumption of three days L-L meal.

连续三天进食L-L的CHO食物，运动后期底物消耗出现了由糖氧化供能转向脂肪氧化供能；连续三天的L-L的CHO食物，能够使运动员在运动中出现良好的腹部舒适状态。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒，并与pCDNA3质粒，按3：1比例混合后，运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO)，用新霉素类似物G418初步筛选出阳性克隆。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Results The value of NAA and Cho/Cr was decreased obviously.The NAA value,between benign and malignant tumor and between intracerebral and extracerebral tumor,had significant effectiveness.But the Cho and Cr value and the peak ratio of metabolites had no significant effectiveness between benign and malignant tumor and between intracerebral and extracerebral tumor.

结果：几乎所有颅脑肿瘤的NAA峰均下降，Cho/Cr下降；良恶性肿瘤之间、脑内外肿瘤之间的NAA峰值的比较差异有显著性，但其Cho、Cr峰值的比较及各代谢产物的峰值比值的比较差异无显著性。

ResultsNo significant changes of NAA/Cr, Cho/Cr and NAA/Cho ratios were observed between the left and right precentral gyrus, nor between the male and female.

结果不同侧别和不同性别之间的NAA/Cr、Cho/Cr和NAA/Cho值均无显著性差异；NAA/Cr与年龄低度负相关，Cho/Cr与年龄低度正相关，NAA/Cho与年龄呈中度负相关。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞，通过潮霉素筛选得到阳性克隆，间接免疫荧光实验鉴定N基因在CHO细胞中的表达，并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达，最终建立了CHO/ CDV-N细胞株，为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

"Cinerin:Either of two compounds, CHO or CHO, used in insecticides."

瓜菊酯：CHO和CHO两种化合物中的任一种，用作杀虫剂。

"Cinerin:Either of two compounds, CHO or CHO, used in insecticide s."

瓜菊酯：CHO和CHO两种化合物中的任一种，用作杀虫剂。

RESULTS: We found that (1) tramadol, similar to the well-knownTRPV1 agonist, capsaicin, significantly increased [Ca2+] i ofTRPV1-CHO cells in a concentration-dependent fashion;(2) itseffect was reversibly prevented by the TRPV1 antagonist capsazepine;(3) repeated application of tramadol resulted in marked tachyphylaxis;and (4) tramadol did not modify [Ca2+] i in control CHO cells.

结果：我们发现以下结果：（1）曲马多和辣椒素这个熟知的辣椒素受体激动剂一样，呈浓度依赖性地显著增加 CHO 细胞 TRPV1内流钙离子；（2）该作用可以被 TRPV1拮抗剂 capsazepine 可逆性地阻止；（3）重复给予曲马多会造成显著的快速耐药性；（4）曲马多不修饰钙对照 CHO 细胞中的内流钙离子。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。