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When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后,同A组相比:E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高,在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著,在卵泡、黄体中阳性产物表达均较A组显著降低,在子宫中表达较A组变化不明显;同B组相比:E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高,在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低;同C组相比:E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高,在室周核、黄体中均显著降低;同D组相比:E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高,在视上核、弓状核显著降低,在其它部位表达变化差异不显著。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果:(1)层粘连蛋白组处于G_2~M期细胞比例较对照组显著提高,Go~G_1期细胞比例显著下降,提示层粘连蛋白促进内皮细胞DNA合成,及细胞分裂增殖;光镜下,对照组细胞分布成团状,细胞密度较低,细胞间连接紧密,细胞内微丝结成网状,细胞核大小一致;与对照组相比,层粘连蛋白组细胞生长旺盛,细胞密度高,向周边伸出大量板状及丝状伪足,细胞内微丝增多、拉长、集结成束,伸入伪足中,细胞核形状大小不一致;(2)倒置显微镜观察及细胞生长曲线显示,各组细胞数目随时间增加而明显增多,各实验组较对照组增生显著,EGF和LN联合应用组各时间点细胞数目最高;实验组处于S期和G_2~M期细胞数目增加,Go~G_1期细胞数目减少;提示EGF、LN单独及联合应用均可促进细胞增殖,但尚不能认为二者有交互作用;(3)倒置显微镜下,组织培养的猫角膜内皮细胞排列成密集的单层,细胞间连接紧密;组织学观察发现,培养的猫角膜内皮细胞形成完整的内皮层,贴附于脱水基质的后弹力膜上,与正常的角膜内皮组织结构相似;扫描电镜下,组织培养的猫角膜内皮细胞间紧密镶嵌排列,可见某些细胞呈近似六边形排列,细胞大小不甚一致,胞核清晰。

Methods: Thirty-six SD rats were injected lipopolysaccharide to form ALI or saline intravenously, then were divided randomly into six groups:(1) Control group;(2) Acute lung injury group;(3) Cytochalasin D group;(4) Phalloidin group;(5) LPS+ Cytochalasin D group;(6) LPS+ Phalloidin group.

方法36只清洁级雄性SD大鼠,静脉注射内毒素复制ALI模型,随机数字法分成6个组:。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment.

在前期预实验的基础上,选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例,按照区组随机原则分为3组,观察组、对照组1、对照组2,每组各20例,采用放射免疫法分别测定各组患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平;通过三组及三组间治疗前后PADAM症状评分指数的对比研究,揭示针药结合的整体调节作用特色,探讨提高针灸疗效的新途径;总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度,探讨针药结合治疗本病的机制及针灸增效剂的应用前景,为今后进一步研究打下坚实基础。

The dose of rHuEPO began to decrease in A group after eighth week, which had distinctive difference with that of B group (P.05). In A group , the dose of rHuEPO was just two thirds of that in B group during the sustain period.6. The kidney function of two group patiences was stable or improved during the treatment period ,which of A group was improved distinctly after treatment.

两组药物都能使患者肾功能好转或稳定,A组治疗后与用药前比较有非常显著性差异(P.01),B组治疗后较治疗前有改善但无统计学意义;治疗结束后两组间比较有显著性差异(P 。05),A组优于B组。

METHODS Thirty six Wistar female rats used in experiment were randomly divided into three groups: the normal control group, 45 d group and 90 d group with the revival liquid of quantum. All rats were administered by free drinking, the normal control group were treated by drinking water in 3 months, 45 d group was performed drinking water in 1.5 months and then revival liquid of quantum in 1.5 months, and the 90 d group was administrated the revival liquid of quantum in 3 months.

方法] 取雌性Wistar大鼠36只,按体重随机分为对照组、量子复活液45 d组和90 d组;以自由饮用方式给药,给予对照组大鼠普通自来水喂养90 d,给予量子复活液45 d组大鼠普通自来水45 d后,再给予量子复活液喂养45 d,给予量子复活液90 d组大鼠量子复活液90 d。

CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.

心肌组织CGRP的含量由正常组、实验对照组至CHF组逐渐升高,差异显著;免疫组化研究表明心肌组织中存在nNOS和CGRP免疫阳性神经细胞及纤维,细胞形态多样,正常多以圆形和椭圆形为主,还有梭形、蝌蚪形、三角形、指环形及不规则形,细胞密度心房高于心室,右心高于左心;CHF组CGRP神经细胞形态有一定改变,淡染的指环形细胞增多,而深染圆形或椭圆形细胞减少,其面、数密度均明显低于正常组,差异显著;用RT-PCR测得CHF组nNOS mRNA极度表达,显著高于正常组,而CGRP表达明显低于正常组。

It is Song Jinghua of 51 years old to be graduated from major of Fudan University business management, came 1976 1994, it is military career 18 years, join in after be transferred to civilian work associate group, have successively held the posts of associate ministry of resource of group labor power vise general manager, associate group limited company vise general manager, associate group limited company general manager, associate general manager of division of group Su Wan, associate ministry of big client of group big China and Russia area is standing vise general manager.

现年51岁的宋京华毕业于复旦大学企业治理专业,1976年至1994年,18年为军人生涯,转业后加盟联想集团,历任联想集团人力资源部副总经理、联想集团有限公司副总经理、联想集团有限公司总经理、联想集团苏皖区总经理、联想集团大中华及俄罗斯区大客户部常务副总经理。

Results ① before the operations, all physical growth characters of the moderate and severe osahs groups were significantly lower than those of the control group, however only the height and sitting height of the mild osahs group were lower than those of the control group.② 6 months after the operations, physical growth characters of the mild and moderate osahs groups were not significantly lower than those of the control group, but some of the severe osahs group still were lower than those of the control group.

结果 术前:中、重度osahs组患儿体格发育指标均低于对照组儿童,轻度osahs组患儿仅身高和坐高低于对照组儿童;术后6个月:轻、中度osahs组患儿体格发育指标与对照组差异无统计学意义,重度osahs组患儿仍有部分体格发育指标低于对照组。

The conclusion to junior high school student"s learning motivation intervention tactics of Group Counseling is: Positive self-suggestion of the member of group; Coming from member of community "important others" encouragement of significant others support; leader of group helps the member of group to improve and study successful understanding on the Basis of WDEP system-, Group"s leader helps the member of group to study and form correct casual attributions cognition.

以团体心理辅导的方法对初中生学习动机的有效干预策略的结论是:团体成员积极的自我暗示;团体领导者收集来自团体成员&重要他人&的鼓励支持;团体领导者依据格拉塞的&WDEP&系统帮助团体成员改善学习成功的认识;团体领导者帮助团体成员对学习形成正确的归因认知;团体领导者为团体成员创设温暖接纳

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