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The levels of SOD and CAT significantly increased and MDA decreased in bifidobacterium group compared to radition damage group.Electronic microgragh of spine neurocyte and neurogliocyte showed that cytoplasm,cytoblast and plastiosome were significantly engorged ,and neurogliocyte apoptosised in earlier period. TLR2mRNA increased in C group compared to B group by RT-PCR. Cytochrome C in kidney increased in C group compared to B group, but bcl-2,NF-Кb decreased. Conclusions : Enteroclysm is better compared to intragastric administration.

双歧杆菌处理组肝组织中SOD和CAT水平明显高于放射损伤组(P.01),同时MDA明显低于放射损伤组(P.01);电镜结果显示:放射损伤组与双歧处理组的细胞浆、细胞核和线粒体均明显肿胀,双歧处理组同时具有早期凋亡现象;RT-PCR结果显示:双歧处理组TLR2mRNA的表达明显高于放射损伤组;免疫组化结果显示,双歧处理组Cytochrome C的表达明显优于放射损伤组,而NF-кBp65和bcl-2则相反。

Methods: 1 To establish rat AS models, rats in the experiment were divided intofive groups: normal control group, hyperlipidemia group (fed with high-cholesterol diet), hyperlipidemia + vitaminDS group, hyperlipidemia + vitamin D3 + endothelium denudate group,losartan group (hyperlipidemia +vitamin D3 + endothelium denudate group treated with losartan).

本实验从AS的主要的三种发病机制出发,建立和比较了三种建立大鼠AS模型的方法;同时以该模型为基础,探讨了MMP-9/TIMP-1的动态平衡与AS的关系;也研究了血管组织及血浆血管紧张素H水平与AS的关系;探讨了AngⅡ 1型受体拮抗剂氯沙坦是否具有抗AS作用,以及机制如何。

It will provide theoretical and experimental base for the research of syndrome of traditional Chinese medicine. Method: 60 depuratory and healthy SD female rats with normal estrous cycle(4~5days),3-month in age, 230±20g in body weight,were divided into 6 groups randomly, normal group,model group,low-dose group,middle-dose group, high dose group,control group.

选用动情周期正常(4~5天)的健康清洁级SD雌性大鼠60只,鼠龄3个月,体重230±20g,随机分为6组,每组10只:即正常对照组,模型对照组,模型加加减温经汤低剂量组,模型加加减温经汤中剂量组,模型加加减温经汤高剂量组(简称为&高剂量组&,低剂量组、中剂量组、高剂量组又合称为&治疗组&),桃红四物汤组。

In Comlex model group the endangium plaque were widespread, while in that of High cholesterol group there was only single small plaque. The pathological heart changes of both High cholesterol group and Complex model group weren't obvious, with mild oedema and small fat drops. The liver cells of High cholesterol group showed mild oedema, while fat drops in the liver of Complex model group were more typical.

病理显示主动脉弓变化明显,复合模型组内膜斑块弥漫,而单纯高脂组内膜只出现单个小斑块,单纯高脂组和复合模型组心脏病变区别不大,均见轻度水肿和小脂肪滴;单纯模型组肝脏细胞轻度水肿,而复合模型组肝脏脂肪滴明显。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment. By the contrast of the grade of PADAM symptom among three groups before and after treatment ,we can reveal the whole funcamental characteristic of acupuncture combined with herb ,and discuss a new way to enhance the curative effect of acupuncture ,and summarize the endocrinous adjustment of acupuncture for the PADAM's patients and the orchidic improvement by the treatment of acupuncture.

在前期预实验的基础上,选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例,按照区组随机原则分为3组,观察组、对照组1、对照组2,每组各20例,采用放射免疫法分别测定各组患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平;通过三组及三组间治疗前后PADAM症状评分指数的对比研究,揭示针药结合的整体调节作用特色,探讨提高针灸疗效的新途径;总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度,探讨针药结合治疗本病的机制及针灸增效剂的应用前景,为今后进一步研究打下坚实基础。

Results:Wound healing rate in group A was higher than that in group B and the wound healing time was 1~3 days earlier in group A.More active proliferation of epidermic cells around wounds showed in group A.The function of fibroblasts in wounds,collagen synthesis and secretion,were more vigorous in group A than those in group B.

结果:A组创面愈合率高于B组,其创面愈合时间较B组提前1~3 d;创周表皮细胞增殖也较B组活跃,创面成纤维细胞较B组呈现出更旺盛的合成与分泌胶原纤维的功能。

The results showed that the perimeter of leg significantly decreased (P.05), further decreased (P.01) in a HDT time-dependent manner compared with pre-HDT; in limb muscular group, the perimeter of leg decreased significantly (P.01) during from the HDT 18th day to the 30th day compared with pre-HDT; in bicycle ergometric group, the perimeter of leg decreased significantly (P.01) during from the HDT 22nd day to the 30th day ; in the HDT 10th day, the perimeter of leg in bicycle ergometric group increased significantly (P.05) as compared to that of control group; in the HDT 18th day, the perimeter of leg in limb muscular and bicycle ergometric group increased significantly (P.05) compared with control group.

结果发现,与卧床前相比,对照组小腿围径在卧床第1d显著减小(P.05),随卧床时间延长进一步显著减小(P.01);下肢肌力训练组在卧床后期(18~30 d)以及起床后的小腿围径显著减小(P.01);自行车功量计训练组在卧床第22~30 d及起床后显著减小(P.01);卧床第10 d自行车功量计训练组的小腿围径显著大于对照组(P.05),第18 d下肢肌力训练组和自行车功量计训练组的小腿围径均显著大于对照组(P.05)。

For those in control group (n=14), the animals did not undergo any surgery of the nerve fibers and ganglia. In Vi group (n=9), the animals underwent bilateral resection of the nasociliary and postganglionic parasympathetic nerve fibbers. In SCG group (n=8), the animals underwent bilateral resection of supper cervical ganglia. In V1+SCG group (n=9), the animals underwent both surgeries as Vi and SCG group did. In sham operation group (n=11), the animals underwent carotid manipulation with blunt-tipped forceps as well as dissection of nasociliary and postganglionic parasympathetic nerve fibers around the ethmoidal foramen, without cutting any nerves. SCS was performed on rats and CBF was monitored by laser Doppler flowmeter recordings.

SD大鼠分5组:对照组(n=14,不对神经纤维和神经节作任何手术处理)、V1组(n=9,切断双侧鼻睫状神经及其副交感节后纤维)、SCG组(n=8,切断双侧颈上神经节)、V1+SCG组(n=9,切断双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节)、假手术组(n=11,手术暴露双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节,但不切断神经纤维或神经节);给予大鼠SCS,并用激光多普勒实时记录脑血流。

Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V1 + SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves.

动物分成5组,对照组:不对神经纤维和神经节作任何手术处理;V1组:切断双侧鼻睫状神经及其副交感节后纤维;SCS组:切断双侧颈上神经节;V1+SCG:切断双侧鼻睫状神经及其副交感节后纤维及双侧颈上神经节;假手术组:手术暴露双侧鼻睫状神经和其副交感节后纤维及双侧颈上神经节,但不切断神经纤维或神经节。

objective observing the clinic effect that curing menopause syndrome with wuzangyangyin soup that self-designed.methods the 120 menopause syndrome patients have divided into two groups randomly,these patints who accorded with the diagnosing standard of the traditional chinese medicine,the cured group giving the self-desingedwuzangyangyin soup,the contrasted group giving "genian an",course of treatment is 1 months.observing the change conditions of effects before cure and after with the two groups,the change conditions include curative effects、symptom score、fsh、e2.results the curative ratio:the cured group having for 10%,the contrasted group being 2.5%.the effective rato:the cured guoup having for 93.7%,the contrasted group being82.5%,with comparing the effect between the two group, the difference is obvious,p.05.conclusion the self-designed"wuzangyangyin soup"has an effect to cure menopause syndrome.

目的 观察五脏养阴汤治疗更年期综合征的临床疗效。方法将符合中医诊断标准的120例更年期综合征患者随机分为两组,治疗组给予自拟五脏养阴汤(由黄精、麦冬、白芍、生地、玉竹、五味子、花粉、丹皮等药物组成),对照组给予更年安治疗,疗程1个月。观察治疗前后两组疗效、症状积分及fsh、e2变化情况。结果治愈率治疗组10.0%,对照组2.5%;总有效率治疗组93.7%,对照组82.5%。两组治愈率及总有效率比较,差异均有显著性(p<0.05)。结论自拟五脏养阴汤治疗更年期综合征有较好的疗效。

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