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Of the group entered the emergency room in 6 hours after injured;(2) In the group, there 21 persons (16.7%) whose AUDIT score were more than 7 and risks were more pronounced for male. 6.3% scored 16 or more. Teetotaller is 36.5% in the group and female is more;(3)In the 126 cases, 15 (11.9%) used alcohol in 6 hours before trauma and 40% of those were binge drinking. Those AUDIT score 7 or more and 16 or more in alcohol-used group were obviously more than those in non-alcohol-used group;(4) In the group, 9 (7.1%) were HED drinkers and 44.4% of those were binge drinking before trauma.

入组病人中92.9%的人能在受伤后6小时内就诊;(2) AUDIT量表总分在7分及以上的21人(16.7%),男性是高危因素,AUDIT量表分≥16分的有8人(6.3%);AUDIT量表分=0的占总数的36.5%,在女性中所占比例较大;(3)入组的病人中有15人(11.9%)在受伤前6小时内有饮酒行为,其中有6人(40%)有狂饮行为,AUDIT量表分≥7分的和≥16分的在有饮酒行为组的比例明显高于无饮酒行为组。

Results: The totally effective rate of treatment group come to 93.3%, while that of control group is 70.0%, and there is a significant difference between the treatment group and control group (P.05). Treatment group can improve significantly the symptoms such as diarrhea, tenesmus, the scorch of anus, bellyache, abdominal distension, and the curative effect is better than that of control group.

结果:临床研究显示,治疗组总有效率93.3%,对照组总有效率70.0%,治疗组总有效率明显优于对照组总有效率(P<0.05);治疗组能明显改善腹泻、里急后重、肛门灼热、腹痛、腹胀等症状,其疗效优于对照组。

INTERVENTIONS: 4 vessel occlusion(4VO) brain ischemic models in rats stained with thionine staining and GFAP immunohistochemistry staining. were used. Sixty-four rats, of which bilateral vertebral arteries were occluded permanently by electrocautery, were divided into the following 8groups: sham operation group, cerebral ischemic preconditioninggroup, ischemic insult group; BIT group; MTPG + sham operation group;MTPG+BIT group; MTPG+ischemia group and -4C3HPG+BIT coup. All the rats were killed 7 days after the operation or the final ischemic treatment. Cerebral sections were selected and stained with thionine staining and GFAP immunohistochemistry staining.

干预:采用大鼠四血管闭塞全脑缺血模型,应用硫堇染色和胶质纤维酸性蛋白免疫组化法。64只大鼠椎动脉凝闭后分为假手术组、单纯预处理组、单纯缺血组、脑缺血耐受组,MTPG+假手术组、MTPG+脑缺血耐受组、MTPG+缺血组和-4C3HPG+脑缺血耐受组,所有动物均在手术后或末次缺血后7 d处死取材,行脑组织切片,硫堇染色和胶质纤维酸性蛋白免疫组化染色。

Result: Scoliosis was identified in 58 cases with Marfan syndrome (38 males and 20 females), the prevalence rate was 42.03%, male-female sex ratio was 1.18:1, 6 cases were in younger than 10-year-old group. 12 cases were in 11~20-year-old group, 19 cases were in 21~30-year-old group, 11 cases were in 31~40-year-old group, 7 cases were in 41~50-year-old group, 3 cases were in 51~60-year-old group. Mean magnitude of Cobb angle in coronal plane was 26.8°±27.8°, the types of scoliosis curve included thoracic curve (36 cases), thoracolumbar curve (11 cases), lumbar curve (2 cases), double curve (6 cases) and triple curve (3 cases), apex vertebraes were convex to the right side among single curves in 38 cases while 11 cases were convex to the left side. Mean magnitude of kyphosis in sagittal plane was 14.3°±13.2°, 5 patients had thoracic lordosis and 40 patients had hypokyphosis and 12 patients had normal kyphosis.

结果:58例(42.03%)患者合并脊柱侧凸,男38例,女20例,男女患病率比例为1.18:1,其中≤10岁6例,11~20岁12例,21~30岁19例,31~40岁11例,41~50岁7例,51~60岁3例;平均冠状面Cobb角为26.8°±27.8°;胸弯36例,胸腰弯11例,腰弯2例,双弯6例,三弯3例;单弯中顶椎凸向右侧38例,凸向左侧11例;矢状面胸椎后凸平均为14.3°±13.2°,其中胸椎前凸5例,胸椎后凸不足40例,胸椎正常后凸12例,仅1例胸椎后凸45°;11例患者冠状面Cobb角>40°,平均年龄15.9岁。

Results The functions of major organs in group HVHF improved obviously,and the MODS morbidity and mortality reduced significantly,compared with that in group HVHF. ENIAF and ECKR combining activity in group HVHF decreased much slowly through the treatment or HVHF than that in group MODS(P 0.01). After hemofiltration the plasma levels of TNF α IL-1β IL-6 IL-8 in group HVHF descended significantly compared with those in group MODS (P.01).Except TNF α, IL-1β.IL-6 IL-8 and IL-10 were detectable in ultrafiltrate.

结果 与MODS组相比,HVHF组各主要脏器功能指标明显改善,MODS的发生率和死亡率显著降低,滤过治疗后ENIAF和ECKR结合活性下降幅度明显趋缓;HVHF组各促炎因子血浆水平明显下降,与MODS组相比有显著差异(P.01);除TNFα外,IL-1β、IL-6、IL-8、IL-10均在超滤液中检测到。

Methods:Unsexed 7-day-old Wistar rats were used as research subjects,and randomly divided into 5 group: the HIBD group(n=12), the HBO early-treated group(n=12), the HBO late-treated group(n=8), the sham operation group(n=12) and the control group(n=6).The HIBD model pups were subjected to unilateral left carotid artery ligation followed by 2h of hypoxia(8%O2 at 37 degree C).

新生7日龄Wistar大鼠为实验动物,雌雄不限,采用结扎左侧颈总动脉后通8%低氧的经典方法制作大鼠缺氧缺血性脑损伤模型,并随机分为HIBD组(n=12)、HBO早治组(n=12)、HBO晚治组(n=8),假手术组(n=12),空白对照组(n=6)。

objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p<0.05/p>0.05),sod was lower than model group(p<0.001) and was higher than control group(p<0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响,探讨其治疗阿尔茨海默病(alzheimer disease,ad)的作用机制。方法用冈田酸(okadaic acid,oa)注射大鼠侧脑室造模,测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p<0.001),高于对照组(p<0.05);sod高于模型组(p<0.001)和对照组(p<0.05);mda低于模型组(p<0.001),高于对照组(p<0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性,降低mda蓄积和tau蛋白生成,具有防治阿尔茨海默病的作用。

130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.

实验方法:将130只Wistar大鼠随机分为五组:正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组,除正常组外,其余各组手术切取坐骨神经4mm后,做坐骨神经硅胶管桥接术,造成周围神经损伤模型,取患侧足三里、三阴交、环跳穴,术后第四天开始电针,每两天一次,每次10分钟,治疗10周后处死动物,取出硅胶管内新生的坐骨神经,纵切片,HE染色、S100免疫组化染色、Sevier-Munger银染,观察坐骨神经纤维再生情况,以健侧同段坐骨神经做对照;取患侧脖肠肌称取湿重,HE染色,观察腓肠肌肌细胞直径,生化测量腓肠肌肌糖元、乳酸。

Methods Thirty-two New Zeeland rabbits were randomly assigned into two groups as experiment group (n=24) end control group (n=8). Each rabbit in experiment group was given injection with 8.0 mg/kg of hydrocortisone acetate for two times per week, while rabbits in control group were given normal saline in same quantity. Serum glucocorticoid concentration, total cholesterol and triglyceride were determined respectively before and 2, 4, 6 and 8 weeks after treatment. Rabbits were sacrificed at the 2nd, 4th, 6th and 8th week. The rabbits, sacrificed at the same time point, were stratified into group A and group B according to the GC concentration, in an attempt to observe the rate of empty bone lacuna and glucocorticoid receptor expression in the soft tissue around hips.

32只新西兰大白兔随机分成两组:实验组(n=24)每周2次肌注醋酸氢化可的松注射液8.0mg/kg造模,对照组(n=8)每周2次肌注等量生理盐水各组于处理的0、2、4、6、8周抽血测定空腹血清GC血药浓度及而清总胆固醇、甘油三脂水平,并于第2、4、6、8周分批处死动物,将同批处死的实验组动物依据血清GC浓度分为A、B两组,观察股骨头的空骨陷窝率及髋周软组织中糖皮质激素受体的表达。

Methods White rabbits were divided into allergosis group and allergosis treated by steroid group. In group A, the model of allergosis was established by intravenous injection of horse serum twice at the interval of 3 weeks. In group B, the model of allergosis was also established by the same method as group A. After 2 weeks, group B was injected with prednisone at 45 mg.kg-1 for 3 days abdominally.

随机分单纯变态反应组和激素治疗变态反应组。A组采用分2次间隔3周给实验白兔iv马血清(10 ml.kg-1)建立变态反应疾病模型。B组采用与A组同样的方法,建立变态反应疾病模型,在模型建立后2周,连续3天腹腔内注射强的松龙(45 mg.kg-1)。

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