查询词典 chlorine group

Methods］ 36 twomonthold rabbits with white feather and black eyes,half male and half female,were randomly divided into 6 groups:normal group,model group,xiangsha liujun pill group, lizhu changle group, lowdose leweiyintreated group,highdose leweiyintreated group.except the normal group, others were made into ibs model of spleen deficiency which was induced by damp heat stress and gavage with fanxieye as well as method of either hunger or gorge.the drug groups were administered preventively from the mediumterm of the model reproduction to the end of modeling.the rabbits of each group were observed.no,nos,mda,sod in serum and sp in colonic membrane were detected.

方法］两月龄白毛黑眼兔36只，雌雄各半，随机分为6组：正常组、模型组、香砂6君丸组、丽珠肠乐组、乐胃饮低剂量组、乐胃饮高剂量组。除正常组外，其余whbe兔采用湿热环境应激联合番泻叶灌胃加饥饱失常复合方法致脾虚泄泻型ibs模型。从模型复制的中期开始各用药组预防给药，至造模结束。观察各组1般情况，测定血清no、nos、mda、sod含量及结肠黏膜组织中sp含量。

Matching facilities: Bed table wide band furniture: Bed, table, wide band repair situation: Good environment: In Zhongqingnalu Haixin plot traffic condition: 12 groups 20 group 208 group 219 group 229 group 303 group 310 group 365 group 365 group 372 groups 37 explained in detail: Four directions Zhongqingnalu in the Haixin plot has two person between hires, the area 16 square meters, the southing, the natural lighting is good, has the kitchen, the bathroom, the wide band, has the direct link municipal government/Taitung/University of Science and Technology/Li Village's vehicle.

个人 配套设施：床桌子宽带家具：床、桌子、宽带装修情况：良好周围环境：重庆南路海信小区内交通状况： 12路 20路 208路 219路 229路 303路 310路 365路 365路 372路 37 详细说明：四方重庆南路海信小区内有双人间出租，面积16平米，南向，采光好，有厨房、卫生间，宽带，有直达市政府/台东/理工大学/李村的车。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Result:1:in the aspect of controlling of the swelling development, treatment group is distinctly superior to control group.2:in the aspect of deswelling rate,treatment group is superior to control group.3:in the aspect of controlling pain degree,shorten the applying time of the anodyne, treatment group is distinctly superior to control group.4:in the aspect of controlling the large-area tension bullae incidence,treatment group is superior to control group.

在消肿速度上，治疗组优于对照组。3。在控制疼痛程度，缩短止痛药应用时间方面，治疗组明显优于对照组。4。在控制大面积张力性水泡发生率上治疗组优于对照组。

Afterwards through the entire audience representative's secret ballot, the selection has produced the human interspace free federation management structure member is the human interspace management group, the group core member name list distinction is as follows: Group leader: Entire unification, authority: Coordinated entire humanity all important business; Military vice-group leader: Yellow Jinlong, authority: Is responsible for entire human all foreign defense business; Lives vice-group leader: Jumbles together, authority: Is responsible to coordinate the humanity to live aspect all business; Produces vice-group leader: White Peter, is responsible to coordinate the human all production activity; Eliminates this is cultural vice-group leader: Black must win, is responsible for the human all culture energetic domain business; Each group is 1000, the prime task assists fellow main members, develops all to serve together the overall humanity's great enterprise!

随后通过全场代表的无记名投票，选取产生了人类星际自由联邦的管理机构成员即人类星际管理小组，小组核心成员名单分别如下：组长：全统一，职权：协调整个人类一切重要事务；军事副组长：黄金龙，职权：负责整个人类一切对外防卫事务；生活副组长：混为一谈，职权：负责协调人类生活方面一切事务；生产副组长：白彼得，负责协调人类一切生产活动；除此就是文化副组长：黑必胜，负责人类一切文化精神领域的事务；各部组员为1000名，主要工作是协助各位主要成员，共同开展一切服务于整体人类的伟大事业！

48 SD rats were randomly di2 vided into eight groups : rest group , exercise group , low pollution exercise group , low pollution rest group , middle pollution exercise group , middle pollution rest group , high pollution exercise group and high pollution rest group .

48只SD雄性大鼠随机分为8组：空白对照组、单纯运动组、低污染运动组、低污染安静组、中污染运动组、中污染安静组、高污染运动组和高污染安静组。

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公司主产品有：K型选粉机、O-Sepa选粉机、组合式选粉机、煤磨动态选粉机、矿渣专用选粉机、粉煤灰专用选粉机、九田立式烘干机、离线脉冲袋式除尘器、砖混结构袋式除尘器、新型行喷脉冲袋除尘器以及其它各类大型布袋和静电除尘等设备6大系列100多个品种，先后承担&十五&省科技工业攻关和国家创新基金项目省重大科技成果转化项目各1个，获国家重点新产品1个，省高新技术产品8个，省火炬计划2个，拥有46项专利，其中1项为国际发明，这些成熟、适用的技术装备已先后为海螺集团、山水集团、华新集团、宜丰盐城、天瑞集团、亚泰集团、金顶集团、安厦集团、江苏长江、磊达集团、越南亚光、孟加拉西隆等2000余家水泥、石膏、钢铁、电力企业服务。

Results By incubating cardiomyocytes with bFGF, ERKs was activated and the cellular viability 〔(70.0±4.6)%in bFGF 10 ng group vs.(53.0±4.5)%in H/R group, P＜0.01） and the ATP content 〔(23.1±2.3) nmol/106 cells in bFGF 10 ng group vs.(12.3±2.1) nmol/106 cells in H/R group, P＜0.01〕 were increased, also the LDH leakage 〔(257.3±51.0) U/L in bFGF 10 ng group vs.(372.5±69.2) U/L in H/R group, P＜0.05〕, was decreased, then attenuation of H/R injury to cardiomyocytes was observed. PD098059, an inhibitor of upstream kinase of ERKs completely abolished the protective effects 〔viability (57.0±5.8)%,ATP content (15.1±2.6) nmol/106 cells,LDH activity in medium (325.5±59.0)U/L in PD098059 group vs. bFGF 10 ng group, P＜0.01, respectively〕.

结果 bFGF激活ERKs，并呈剂量依赖性减轻H/R所致心肌细胞损伤，表现为细胞存活率〔bFGF 10 ng组(70.0±4.6)%，H/R组(53.0±4.5)%，P＜0.01〕和细胞内ATP含量〔bFGF 10 ng组(23.1±2.3)nmol/106细胞，H/R组(12.3±2.1)nmol/106细胞，P＜0.01〕升高，细胞浆酶LDH漏出〔bFGF 10 ng组（257.3±51.0）U/L，H/R组（372.5±69.2）U/L，P＜0.05〕减少；ERKs上游激酶抑制剂PD098059完全消除上述保护作用〔PD098059组细胞存活率（57.0±5.8）%，ATP含量（15.1±2.6）nmol/106细胞，培养液中LDH活性（325.5±59.0）U/L，与bFGF 10 ng组比较均为P＜0.01〕。

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Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48