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Eleven subcloned DNA fragments from the 5′- upstream region of lipA, lipC and lipF of Phanerochaete chrysosporium were assayed by using the gel mobility shift assay. The total proteins extracted from P.chrysosporium mycelia grown in Kirk medium and natural fir wood chip were used to identify the segments in these 11 DNA fragments which are controlled by some regulatory proteins.

用DNA-蛋白质体外结合实验和凝胶迁移率变动分析技术筛选黄孢原毛平革菌木质素过氧化物酶基因lipA、lipC、lipF的5′-端调控区内能与该菌在木质素降解条件下形成的蛋白特异结合的顺式作用元件。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台盼蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

In the first part, it describe the two-level system with the quantum mechanics which include the dress state picture, the adiabatic description of the interaction and the mathematic method; In the second part, it tell us that using the shaping-pulse how to manipulate the quantum coherent control, chip field and tanh field are invested.

第一部分二能级系统的量子描述在这一部分中详细推导了缀饰态表象中量子态的描述,重点介绍了绝热条件下的布洛赫矢量的表达式,以及求解布洛赫矢量的方法。

After Bain Capital announced its plans to buy 3Com in September 2007, the complaint alleges, Mr. Goffer broke a disposable cellphone in half and bit its 'SIM' computer-chip before telling an unidentified 'tippee' to dispose of the other half of the phone.

起诉书指控说,在贝恩资本于2007年9月宣布了收购3Com的计划后,高佛尔将一部一次性使用手机折成两半,并咬断了这部手机的SIM卡,并告知一个身份不明的&内幕消息获得者&处理掉这部手机的另一半。

They have been demonstrated to be used in analysis of most failures in flip chip assembly as follows: 1 We firstly reported the IRM inspection of underfill delamination and crack.

主要结果为: 1首次利用红外显微镜测出了倒装芯片中的下部填充料裂缝和分层的位置,以及分层中空气间隙的大小。

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

设计1组特殊荧光标记探针以构建1种检测p16基因启动子区3个CpG位点甲基化改变的芯片,特殊荧光标记探针包括1对非甲基化探针和甲基化探针,检测相邻的3个CpG位点甲基化的程度。

Objective: To study the expression of osteopontin in oral verrucous carcinoma and oral squamous cell carcinoma, so as to investigate the role of this gene in the development of OVC and OSCC, and to validate the results of gene chip.

目的研究骨桥蛋白(osteopontin,OPN)在口腔疣状癌和口腔鳞癌中的表达,探讨其在口腔疣状癌和口腔鳞癌发生发展中的作用,对基因芯片结果进行验证。

In this paper,the necessity of frequency tracking in ultrasonic cleaning equipmens is discussed.Based on introduction on the principle of the mian circuit of the ultrasonic power supply for cleaning equipmens and the principle of PLL,the output frequency of 74HC4046 is changed by a single chip measuring the phase difference between volage and current.The software of adaptive tracking technique with varied frequency steps is completed.

讨论了超声波清洗机中自动频率跟踪的必要性,在介绍超声波清洗机的主电路原理、PLL锁相环原理的基础上,利用单片机检测超声电源负载的电流、电压的相位差,从而改变74HC4046 的输出频率,完成了变步长频率跟踪软件设计。

Despite the strong impetus in advancement of fluid drives for microfluidic system in the past decade, as evidenced by the multitude of micropumps developed, integration of driving systems on the microfluidic chip as well as the development of reliable microfluid driving systems still remained to be a challenge.

尽管微流体驱动技术在过去的十余年得到了飞速发展,各种微泵种类繁多,但微流体驱动系统集成化和微流体驱动系统可靠性的提高仍然是微全分析系统的薄弱环节。

They also have fish and chip shops in Australia,but there are not so many in the USA.

在澳大利亚的店里,也有鱼和炸土豆条,但是在美国就没有这么多了。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。